Modulation of nitric oxide and 6-keto-prostaglandin F(1alpha) production in bovine aortic endothelial cells by conjugated linoleic acid

Endothelium : Journal of Endothelial Cell Research
Paul M CoenP A Cahill

Abstract

Conjugated linoleic acid (CLA) refers to a group of polyunsaturated fatty acids that exist as positional (18:2) and stereo (cis/trans) isomers of conjugated dienoic octadecadienoate. Reports consistently indicate that CLA may inhibit both the onset and progression of atherosclerosis, via an as yet unknown mechanism(s). In an effort to identify the putative biochemical effects of CLA on bovine aortic endothelial cells (BAECs), the authors examined both the temporal and dose-dependent effects of a commercial CLA isomeric mixture on the expression and enzymatic function of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-I/II (COX-I/II) in these cells. Initial investigations indicated that CLA mix (0 to 10 microg/mL, 0 to 24 h) failed to regulate either the expression or activity of eNOS in BAECs under basal conditions. Pretreatment of BAECs with CLA mix (10 microg/mL) for either 3 or 24 h, followed by incubation with 5 microM bradykinin (BK) for 3 h, however, increased BK-stimulated nitrite release by 2.4 +/- 0.6- and 3.0 +/- 0.4-fold, respectively, more than control cells (BK-stimulation without CLA pretreatment). Under basal conditions, CLA mix (10 microg/mL, 0 to 24 h) had no significant effect on either COX-I or CO...Continue Reading

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Citations

Jul 23, 2009·The British Journal of Nutrition·Robert Ringseis, Klaus Eder
Aug 8, 2006·European Journal of Pharmacology·Guangming LiMark E Cook
Mar 9, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Robert Ringseis, Klaus Eder
May 26, 2009·The International Journal of Biochemistry & Cell Biology·Paul A FitzpatrickPhilip M Cummins
Jan 1, 2008·Nutrition Research and Practice·Yunkyoung Lee
Sep 5, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Philip M CumminsPaul A Cahill
Jul 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Nicholas P TobinPhilip M Cummins
Apr 28, 2011·Journal of Animal Science·B R WiegandF C Parrish
Aug 27, 2021·Prostaglandins & Other Lipid Mediators·Mibsam M RojasHugo S García

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