PMID: 7540707Jan 1, 1995Paper

Modulation of nitric oxide-dependent relaxation of pig tracheal smooth muscle by inhibitors of guanylyl cyclase and calcium activated potassium channels

Life Sciences
M S Kannan, D E Johnson

Abstract

Nitric oxide is released from intrinsic nonadrenergic, noncholinergic (NANC) nerves of pig tracheal smooth muscle (TSM) in response to electrical field stimulation (EFS). In this study, we investigated the role of guanylyl cyclase in the NANC relaxation by using guanylyl cyclase inhibitors, LY83583 and methylene blue (MB). The role of large conductance calcium-activated potassium (KCa) channels in mediating NANC relaxation was studied by using inhibitors of this channel, charybdotoxin and iberiotoxin. In carbachol-contracted TSM strips, LY83583 (10-20 microM) and MB (10-100 microM) resulted in inhibition of EFS-induced relaxations at all frequencies studied. Relaxations induced by exogenous 8-Bromo-cyclic 3',5'-guanosine monophosphate (8-Br-cGMP) were unaffected by LY83583. The concentration-relaxation curves to isoproterenol, which acts by elevating adenosine-3',5'-cyclic monophosphate (cAMP), and the nitric oxide donors sodium nitroprusside (SNP) or S-nitroso-n-acetylpenicillamine (SNAP) were unaffected by LY83583. Both charybdotoxin (240 nM) and iberiotoxin (180 nM) attenuated relaxations induced by EFS and SNAP. The role of guanylyl cyclase activation in the relaxation to EFS of pig TSM is suggested by the sensitivity of th...Continue Reading

References

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Citations

Jul 27, 2000·Journal of Cellular Physiology·J A CarvajalC P Weiner
Apr 20, 2000·Indian Journal of Pediatrics·M S KannanD E Johnson
Jun 25, 1997·European Journal of Pharmacology·I G Johansson RydbergM Grenegård
Aug 11, 1998·European Journal of Pharmacology·P RecioA García-Sacristán
Jan 1, 1997·Respiration Physiology·J S ShenbergerM S Kannan
Sep 7, 2006·Nature Reviews. Drug Discovery·Oleg V EvgenovJohannes-Peter Stasch
Apr 16, 1998·British Journal of Pharmacology·M HernándezU Simonsen
Jun 18, 1998·British Journal of Pharmacology·K A KuenzliM E Bradley
Oct 4, 2000·British Journal of Pharmacology·F Lovren, C Triggle
Jun 29, 1999·Journal of Autonomic Pharmacology·M V BarahonaM San Andrés
Oct 1, 1996·British Journal of Pharmacology·K A KuenzliI L Buxton
Oct 23, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Praveen ShuklaStephen T O'Rourke
May 26, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Marc ChesterSteven H Abman
Sep 9, 1998·American Journal of Respiratory Cell and Molecular Biology·A AbderrahmaneE Rousseau
Nov 11, 2008·Reproduction in Domestic Animals = Zuchthygiene·M ChruścielA Postek
Dec 24, 2003·Autonomic & Autacoid Pharmacology·C H JunU D Sohn
Jul 28, 2004·Biochemical Pharmacology·Lérida Guerra de GonzálezMarcelo J Alfonzo
Sep 8, 2016·Pulmonary Pharmacology & Therapeutics·Vítor S FernandesMedardo Hernández
Jul 23, 2004·Physiological Reviews·Fabio L M RicciardoloGert Folkerts
Apr 12, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Deepak A DeshpandeMathur S Kannan
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Thomas A WhiteMathur S Kannan
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adriana CatalliLuke J Janssen
Jan 2, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthias SausbierPeter Ruth
Aug 23, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Marc ChesterSteven H Abman
Jun 1, 1997·Journal of Applied Physiology·Y S PrakashG C Sieck
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joris KloekGert Folkerts
Dec 18, 2002·Experimental Biology and Medicine·Jong Kwan ParkKyung Woo Cho

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