Modulation of O-GlcNAc glycosylation during Xenopus oocyte maturation

Journal of Cellular Biochemistry
Tony LefebvreJean-Pierre Vilain

Abstract

O-linked N-acetylglucosamine (O-GlcNAc) glycosylation is a post-translational modification, which is believed antagonises phosphorylation. We have studied the O-GlcNAc level during Xenopus oocyte meiotic resumption, taking advantage of the high synchrony of this model which is dependent upon a burst of phosphorylation. Stimulation of immature stage VI oocytes using progesterone was followed by a 4.51 +/- 0.32 fold increase in the GlcNAc content, concomitantly to an increase in phosphorylation, notably on two cytoplasmic proteins of 66 and 97 kDa. The increase of O-GlcNAc for the 97 kDa protein, which we identified as beta-catenin was partly related to its accumulation during maturation, as was demonstrated by the use of the protein synthesis inhibitor--cycloheximide. Microinjection of free GlcNAc, which inhibits O-glycosylated proteins-lectins interactions, delayed the progesterone-induced maturation without affecting the O-GlcNAc content. Our results suggest that O-GlcNAc glycosylation could regulate protein-protein interactions required for the cell cycle kinetic.

References

Jan 1, 1991·Genes & Development·H Ellinger-Ziegelbauer, C Dreyer
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K P Kearse, G W Hart
May 1, 1987·The Journal of Cell Biology·C M SnowL Gerace
Feb 13, 1997·Biochemical and Biophysical Research Communications·R S HaltiwangerR Scartozzi
May 1, 1997·Molecular and Cellular Biology·I Han, J E Kudlow
May 1, 1997·Developmental Biology·C M HedgepethP S Klein
Jul 1, 1997·The EMBO Journal·H AberleR Kemler
Oct 6, 1997·The Journal of Biological Chemistry·K OrfordS W Byers
Oct 29, 1997·Molecular and Cellular Biology·M D RoosJ E Kudlow
Jan 1, 1995·Progress in Cell Cycle Research·Y Gotoh, E Nishida
Jun 22, 1999·Archives of Biochemistry and Biophysics·M W MillerJ A Hanover
Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J E Ferrell
Nov 26, 1999·Biochimica Et Biophysica Acta·T LefebvreJ C Michalski
Aug 5, 2000·The Journal of Biological Chemistry·F I Comer, G W Hart
Aug 26, 2000·Cytokine & Growth Factor Reviews·T Akiyama
Nov 7, 2000·Current Opinion in Cell Biology·A R Nebreda, I Ferby
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Keith VossellerGerald W Hart

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Citations

Jul 18, 2008·Glycoconjugate Journal·Vanessa DehennautJean-François Bodart
Jan 31, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Natasha E Zachara
Apr 23, 2009·BMC Developmental Biology·Danielle M WebsterScott T Dougan
Sep 1, 2014·Amino Acids·Alexis K Nagel, Lauren E Ball
Jul 12, 2008·Molecular & Cellular Proteomics : MCP·Vanessa DehennautTony Lefebvre
Apr 15, 2017·Biochemical Society Transactions·Ee Phie TanChad Slawson
Dec 1, 2017·Malaria Journal·Mattis KupferschmidTony Lefebvre
Mar 3, 2007·The Journal of Biological Chemistry·Vanessa DehennautJean-François Bodart
May 26, 2011·Médecine sciences : M/S·Stéphanie OlivierTony Lefebvre
Nov 29, 2014·Proteomics·Anne-Sophie Vercoutter-EdouartTony Lefebvre
Apr 20, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stéphanie Olivier-Van StichelenTony Lefebvre
Aug 15, 2018·Frontiers in Endocrinology·Caifei Liu, Jing Li
Nov 25, 2011·American Journal of Physiology. Endocrinology and Metabolism·Stéphanie Olivier-Van StichelenTony Lefebvre
Jun 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Viktória FisiTamás Nagy
Feb 23, 2019·Molecular Reproduction and Development·Luhan T ZhouFrancesca E Duncan
Jul 13, 2021·Frontiers in Chemistry·Qiang Zhu, Wen Yi
Sep 29, 2005·Biochemical and Biophysical Research Communications·Kyung-Cheol Sohn, Su-Il Do
Jun 20, 2006·Biochimica Et Biophysica Acta·Natasha E Zachara, Gerald W Hart

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