Modulation of outer hair cell electromotility by cochlear supporting cells and gap junctions.

PloS One
Ning Yu, Hong-Bo Zhao

Abstract

Outer hair cell (OHC) or prestin-based electromotility is an active cochlear amplifier in the mammalian inner ear that can increase hearing sensitivity and frequency selectivity. In situ, Deiters supporting cells are well-coupled by gap junctions and constrain OHCs standing on the basilar membrane. Here, we report that both electrical and mechanical stimulations in Deiters cells (DCs) can modulate OHC electromotility. There was no direct electrical conductance between the DCs and the OHCs. However, depolarization in DCs reduced OHC electromotility associated nonlinear capacitance (NLC) and distortion products. Increase in the turgor pressure of DCs also shifted OHC NLC to the negative voltage direction. Destruction of the cytoskeleton in DCs or dissociation of the mechanical-coupling between DCs and OHCs abolished these effects, indicating the modulation through the cytoskeleton activation and DC-OHC mechanical coupling rather than via electric field potentials. We also found that changes in gap junctional coupling between DCs induced large membrane potential and current changes in the DCs and shifted OHC NLC. Uncoupling of gap junctions between DCs shifted NLC to the negative direction. These data indicate that DCs not only pr...Continue Reading

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Sep 2, 2010·Purinergic Signalling·Yan Zhu, Hong-Bo Zhao

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Citations

May 14, 2011·Journal of the Association for Research in Otolaryngology : JARO·Christopher C LiuJohn S Oghalai
Sep 2, 2010·Purinergic Signalling·Yan Zhu, Hong-Bo Zhao
May 9, 2013·Nature Communications·Yan ZhuHong-Bo Zhao
Sep 18, 2012·Biochemical and Biophysical Research Communications·Yan Zhu, Hong-Bo Zhao
Jun 16, 2015·Frontiers in Cellular Neuroscience·Jeffrey C Wingard, Hong-Bo Zhao
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrei N LukashkinGuy P Richardson
Jun 21, 2019·International Journal of Molecular Sciences·László KölesTibor Zelles
Jun 14, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Rui YeJianxiong Li
Dec 18, 2010·The Journal of Biological Chemistry·Snezana Levic, Ebenezer N Yamoah
Sep 8, 2017·Frontiers in Cellular Neuroscience·Shimon P Francis, Lisa L Cunningham
Jun 25, 2019·Hearing Research·Karl D LerudEdward W Large
Jun 7, 2011·Biophysical Journal·Stefan JacobAnders Fridberger

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