Modulation of P-selectin expression in the postischemic intestinal microvasculature

The American Journal of Physiology
M J EppihimerD Neil Granger

Abstract

The dual radiolabeled monoclonal antibody technique was used to 1) define the magnitude and kinetics of P-selectin expression in murine small intestine exposed to ischemia-reperfusion (I/R), and 2) determine the factor(s) responsible for initiating this response. Within 10 min after release of a 20-min arterial occlusion, intestinal P-selectin expression increased two- to threefold compared with control values. Peak (4-fold) expression of P-selectin was noted at 5 h after reperfusion, returning to the control value at 24 h. The early (10-30 min) I/R-induced upregulation of P-selectin appears to reflect mobilization of a performed pool of the adhesion molecule, whereas the later (5 h) rise appears to be transcription dependent. The early increase in P-selectin expression was not inhibited by pretreatment with either oxypurinol (inhibits xanthine oxidase), diphenhydramine (H1-receptor antagonist), or MK-571 (leukotriene C4/D4 antagonist), nor was it blunted in transgenic mice expressing three times the normal level of copper-zinc superoxide dismutase or in mast cell-deficient mice. However, significant inhibition was noted after treatment with either MK-886 (5-lipoxygenase inhibitor) or a nitric oxide (NO) donor (diethylenetriami...Continue Reading

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Citations

Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·F M AkgürD N Granger
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Alyson J ProrockKlaus Ley
Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Paul KubesRichard C Woodman
Feb 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·C W LushP R Kvietys
Apr 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T J VachharajaniD N Granger
Jun 22, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·J RussellD N Granger
May 9, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dianne CooperD Neil Granger
Jul 7, 2009·The Journal of Biological Chemistry·Johanna HolInger Oynebråten
Nov 20, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mikiji MoriD Neil Granger
Jan 21, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·C AnthoniC F Krieglstein
Aug 9, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Hong YuRonald J Korthuis
Dec 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Abdallah GendyMohamed Kotb El-Sayed

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