PMID: 9536430Apr 16, 1998Paper

Modulation of peptide-dependent allospecific epitopes on HLA-DR4 molecules by HLA-DM

Human Immunology
S DroverG T Nepom

Abstract

Peptide binding to HLA-DR molecules in intracellular compartments is facilitated by HLA-DM molecules, present in most types of antigen-presenting cells. Allorecognition of DR specificities represents a form of T cell recognition of the MHC-peptide complex which in some cases is influenced by peptide binding. DRA and DRB*0401 (Dw4) genes were introduced into different cell types including DM-negative and DM-restored mutant cells to analyze recognition of DR4 subtypes by alloreactive T cell clones and Dw4-specific monoclonal antibodies. Distinct patterns of T cell recognition were identified: (i) deficient response to Dw4 molecules in the absence of DM expression in which T cell responses were restored by transfecting DM into the Dw4-expressing cells; and (ii) equivalent recognition of Dw4 on DM- and DM+ cells. Using several mAb to Dw4 molecules, a similar distinction was observed: a shared epitope on Dw4 and Dw14 molecules was partially DM-independent while a Dw4-specific epitope was DM-dependent and cell type-specific. Thus, a subset of both T cell and mAb allodeterminants are influenced by a DM-dependent interaction of MHC molecules with peptides, while the formation of DM-independent allodeterminants may represent direct MHC ...Continue Reading

References

Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G LombardiR I Lechler
Jan 1, 1985·Immunogenetics·R D SalterP Cresswell
Jul 1, 1995·Immunology Today·A BenhamJ Neefjes
Jun 29, 1995·Nature·V S SloanD M Zaller
May 1, 1995·The Journal of Experimental Medicine·J HammerF Sinigaglia
Jan 1, 1995·Immunogenetics·H G RammenseeS Stevanoviíc
Jul 1, 1993·The Journal of Experimental Medicine·R M ChiczJ L Strominger
Mar 1, 1996·Science·W P Fung-LeungL Karlsson
Feb 1, 1996·Current Opinion in Immunology·R Busch, E D Mellins
Mar 1, 1997·International Immunology·D C Douek, D M Altmann

❮ Previous
Next ❯

Citations

Feb 10, 2009·Immunologic Research·Jason M Weaver, Andrea J Sant
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N S PatilE D Mellins
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·John D LichJanice S Blum
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·N S PatilE D Mellins

❮ Previous
Next ❯

Related Concepts

Related Feeds