Modulation of PGC-1 coactivator pathways in brown fat differentiation through LRP130.

The Journal of Biological Chemistry
Marcus P CooperBruce M Spiegelman

Abstract

The PGC-1 coactivators are important regulators of oxidative metabolism. We previously demonstrated that LRP130 is a binding partner of PGC-1alpha, required for hepatic gluconeogenesis. LRP130 is the gene mutated in Leigh syndrome French Canadian variant, a rare neurodegenerative disease. The importance of LRP130 in other, non-hepatocyte biology remains obscure. To better understand PGC-1 coactivator function in brown fat development, we explored the metabolic role of LRP130 in brown adipocyte differentiation. We show that LRP130 is preferentially enriched in brown fat compared with white, and induced in a PGC-1-dependent manner during differentiation. Despite intact PGC-1 coactivator expression, brown fat cells deficient for LRP130 exhibit attenuated expression of several genes characteristic of brown fat, including uncoupling protein 1. Oxygen consumption studies support a specific defect in proton leak due to attenuated uncoupling protein 1 expression. Notably, brown fat cell development common to both PGC-1 coactivators is governed by LRP130. Conversely, the cAMP response controlled by PGC-1alpha is not regulated by LRP130. These data implicate LRP130 in brown fat cell development and differentiation.

References

Jan 16, 1978·European Journal of Biochemistry·G M HeatonD G Nicholls
Dec 6, 1976·Biochemical and Biophysical Research Communications·D Ricquier, J C Kader
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F BouillaudJ Weissenbach
Dec 1, 1965·The Journal of Physiology·D Hull, M M Segall
Jan 1, 1981·European Journal of Applied Physiology and Occupational Physiology·P HuttunenV Kinnula
Apr 1, 1982·The Journal of Clinical Endocrinology and Metabolism·D RicquierG Mory
Dec 13, 2000·Molecular and Cellular Biology·M FasshauerC R Kahn
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Apr 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Johannes KleinC Ronald Kahn
Jan 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vamsi K MoothaEric S Lander
Aug 1, 1964·The Journal of Physiology·M J DAWKINS, D HULL
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Feb 18, 2004·Molecular and Cellular Biology·Yu-Hua TsengC Ronald Kahn
Apr 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Vamsi K MoothaBruce M Spiegelman
Aug 11, 2006·The Biochemical Journal·Jacob B Hansen, Karsten Kristiansen
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Junichiro SonodaRonald M Evans
Jul 10, 2007·Cell Metabolism·Patrick SealeBruce M Spiegelman
Nov 2, 2007·The EMBO Journal·Evangelos KiskinisMalcolm G Parker

❮ Previous
Next ❯

Citations

May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Masanobu KawaiClifford J Rosen
Mar 12, 2010·The Journal of Biological Chemistry·Vishal M GohilVamsi K Mootha
Oct 6, 2011·The Journal of Biological Chemistry·Lijun LiuMarcus P Cooper
Mar 5, 2010·Molecular Biology of the Cell·Florin SasarmanUNKNOWN LSFC Consortium
Aug 6, 2011·Journal of Atherosclerosis and Thrombosis·Pin-I HuangChung-Lan Kao
Jan 17, 2014·The Journal of Clinical Investigation·Lijun LiuMarcus P Cooper
Oct 30, 2015·Brain : a Journal of Neurology·Monika OláhováRobert W Taylor
Jul 17, 2010·Biochemical and Biophysical Research Communications·Fredrik H SterkyNils-Göran Larsson
May 31, 2012·The Biochemical Journal·Joanna Rorbach, Michal Minczuk
Nov 5, 2011·Biochimica Et Biophysica Acta·Oliver Rackham, Aleksandra Filipovska
Dec 19, 2017·Nucleic Acids Research·Michal Gavish-IzaksonYosef Shiloh
Jun 4, 2019·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Hashim IslamBrendon J Gurd
Feb 18, 2020·Pflügers Archiv : European journal of physiology·Hashim IslamBrendon J Gurd
Nov 19, 2015·American Journal of Physiology. Endocrinology and Metabolism·Philip HallenborgKarsten Kristiansen
Mar 4, 2017·Virchows Archiv : an International Journal of Pathology·Samatha BhatKapaettu Satyamoorthy
Jun 6, 2019·International Journal of Molecular Sciences·Katya Vargas-OrtizMaciste H Macías-Cervantes
Oct 4, 2020·Phytochemistry·Bilal AhmadEng Hwa Wong
Nov 29, 2020·Molecular Metabolism·Subramanian SenthivinayagamNorbert Leitinger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Cachexia

Cardiac cachexia is a syndrome associated with the progressive loss of muscle and fat mass. It most commonly affects patients with heart failure and can significantly decrease the quality of life and survival in these patients. Here is the latest research on cardiac cachexia.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.