PMID: 7019376Apr 1, 1981Paper

Modulation of phagosome-lysosome fusion in mouse macrophages

The Journal of Experimental Medicine
M C Kielian, Z A COHN

Abstract

A previously described fluorescence assay has been used to characterize factors that modulate phagosome-lysosome (P-L) fusion in mouse macrophages. Fusion was not affected by enzymatic modification or by concanavalin A cross-linking of the plasma membrane or by coating the phagocytic particle with concanavalin A or immune serum. Pretreatment of cells with 10-5-10-4 M colchicine, or treatment immediately after ingestion with 1-10 microgram/ml cytochalasin did not alter P-L fusion; implying that the cytoskeleton does not control fusion in a rate-limiting way. Fusion was strikingly elevated in 5-h cultures of activated macrophages from immune-boosted mice. A lower enhancement was seen in cells activated by proteose-peptone, a nonspecific inflammatory agent.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E M MahoneyZ A Cohn
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Citations

Jun 28, 2000·Vaccine·L A HilgersB Boon
Jan 1, 1983·The Journal of Cell Biology·R M SteinmanZ A Cohn
Jul 1, 1981·The Journal of Experimental Medicine·M C Kielian, Z A Cohn
Jan 1, 1994·The Journal of Experimental Medicine·R M Steinman, C L Moberg
Apr 1, 1987·Collagen and Related Research·V Everts, W Beertsen
Apr 1, 1983·Experimental and Molecular Pathology·G A Warr, G J Jakab
Dec 1, 1982·Scandinavian Journal of Immunology·E JohnsonR Seljelid
Dec 1, 1988·Kisaengch'unghak chapchi. The Korean journal of parasitology·Won Young ChoiJae Eul Yoo
Dec 16, 1998·The Journal of Biological Chemistry·C Alvarez-Dominguez, P D Stahl

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