PMID: 11319161Apr 25, 2001Paper

Modulation of potassium current characteristics in human myometrial smooth muscle by 17beta-estradiol and progesterone

Biology of Reproduction
G A KnockPhilip Irving Aaronson

Abstract

The K(+) channel currents are important modulators of smooth muscle membrane potential and excitability. We assessed whether voltage-gated K(+) currents from human myometrium are regulated by placental steroid hormones during pregnancy and labor. Pregnant human myometrial cells were isolated from samples obtained at cesarean section. Primary cultured cells were treated with 100 nM 17beta-estradiol, 1 microM progesterone, or both hormones in combination for 24 h. Acute effects of the two hormones were also determined. The K(+) currents were recorded using the standard whole-cell, patch-clamp technique. Primary cultures possessed both delayed rectifier (I(KV)) and A-like (I(KA)) voltage-gated K(+) currents. The 24-h 17beta-estradiol treatment caused a hyperpolarizing shift in the steady-state inactivation of both I(KV) and I(KA). Progesterone treatment also shifted the inactivation of I(KA) and increased I(KV) amplitude by 60%-110%. Conversely, the combined treatment had no effect on these currents. Neither 17beta-estradiol (0.1-1 microM) nor progesterone (1-5 microM) had any effect on the K(+) current when applied acutely. These results show that 17beta-estradiol should inhibit myometrial K(+) channel activity, whereas progester...Continue Reading

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Citations

Aug 19, 2008·Pflügers Archiv : European journal of physiology·Laura A McCallumRachel M Tribe
May 5, 2005·Experimental Neurology·Adrian SculptoreanuLori A Birder
Nov 6, 2007·Reproductive Biology and Endocrinology : RB&E·Ryan C SmithMichael E Bradley
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Mar 10, 2015·Molecular Human Reproduction·R NovakovićL J Gojković-Bukarica
May 13, 2003·Reproductive Biology and Endocrinology : RB&E·Yan LiLisa C Freeman
Sep 13, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Kenjiro SawadaYoshihisa Kurachi
Jul 29, 2011·Reproductive Sciences·Melvyn S SoloffSarah K England
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Feb 13, 2020·Reproductive Sciences·Marina IlicicJonathan W Paul
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Jun 29, 2007·Seminars in Cell & Developmental Biology·Adam M BrainardSarah K England
Dec 2, 2017·Seminars in Perinatology·Joshua D YoungerGeorge Gallos

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