Modulation of pre- and postsynaptic calcium dynamics by ionotropic glutamate receptors at a plastic synapse

Journal of Neurophysiology
N E Schwartz, S Alford

Abstract

This study was conducted to assess the role of ionotropic glutamate receptors in the modulation of calcium dynamics on both sides of a vertebrate plastic synapse. Retrograde labeling of neuronal elements with high-affinity calcium-sensitive dyes was used in conjunction with confocal imaging techniques in an in vitro lamprey brain stem preparation. A prolonged calcium transient was measured both pre- and postsynaptically in response to a period of high-frequency ("tetanic") stimulation to the vestibulospinal-reticulospinal synapse. The ionotropic glutamate receptor antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (10 microM) and D,L-2-amino-5-phosphonopentanoate (D,L-AP5; 100 microM) reduced the calcium signal in both compartments of the synapse. The presynaptic D,L-AP5-sensitive component was enhanced markedly by the removal of Mg2+ from the superfusate. Increasing the extracellular stimulus intensity progressively augmented the presynaptic calcium signal, suggesting the recruitment of excitatory axo-axonic inputs onto these fibers. Further, the presence of an excitatory amino acid-mediated presynaptic potential underlying a component of the Ca2+ signal was demonstrated by electrophysiological recordings from vestibulospinal ax...Continue Reading

References

Aug 1, 1977·The Journal of Physiology·W O Wickelgren
May 1, 1992·The Journal of Physiology·E Neher, G J Augustine
May 1, 1992·The Journal of Physiology·G J Augustine, E Neher
Jan 1, 1991·Annals of the New York Academy of Sciences·G J AugustineM P Charlton
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T J O'DellO Arancio
Oct 15, 1991·Biochemical and Biophysical Research Communications·M Eberhard, P Erne
Aug 23, 1990·Nature·J M Bekkers, C F Stevens
Sep 22, 1989·Science·A IrikiH Asanuma
Aug 1, 1989·Neuron·L O Trussell, G D Fischbach
Apr 7, 1987·Brain Research·J T BuchananS Grillner
Dec 1, 1993·Trends in Neurosciences·R C Malenka, R A Nicoll
Jan 7, 1993·Nature·T V Bliss, G L Collingridge
Mar 1, 1997·The Journal of Physiology·A J Cochilla, S Alford

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Citations

May 10, 2008·Nature Reviews. Neuroscience·Paulo S Pinheiro, Christophe Mulle
Dec 18, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Susan M BarmanGerard L Gebber
Feb 26, 2011·The International Journal of Neuroscience·Dimitrios MichmizosStavros Baloyannis

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