Modulation of rabbit reticulocyte guanine nucleotide exchange factor activity by casein kinases 1 and 2 and glycogen synthase kinase 3

Biochemistry
L P SinghA J Wahba

Abstract

The in vitro phosphorylation of the guanine nucleotide exchange factor (eIF-2B) by casein kinase 2 (CK-2) was previously shown to stimulate the binding of GTP to eIF-2B and increase nucleotide exchange [Singh, L. P., Aroor, A. R., & Wahba, A. J. (1994) Biochemistry 33, 9152-9157]. The present study examines the in vitro phosphorylation of the 82-kDa subunit of eIF-2B by CK-1 and glycogen synthase kinase 3 (GSK-3) and the effects of this covalent modification on nucleotide exchange. Phosphorylation with CK-1 adds approximately 0.27 mol of phosphate/mol of eIF-2B and doubles guanine nucleotide exchange activity. Treatment of the phosphorylated eIF-2B with alkaline phosphatase reduces its activity by a factor of 4, and rephosphorylation with CK-1 (0.49 mol of phosphate/mol of eIF-2B) restores its specific activity to that of the phosphorylated protein. GSK-3 phosphorylates the 82-kDa subunit of both isolated and alkaline phosphatase-treated eIF-2B; however, the stoichiometry of phosphorylation is much less (approximately 0. 12 mol/mol of eIF-2B in both preparations) than that obtained with CK-1 or CK-2. Phosphorylation of eIF-2B with GSK-3 neither stimulates nor inhibits GDP/GTP exchange. The results of this study indicate that ph...Continue Reading

References

Dec 30, 1987·European Journal of Biochemistry·M PerezE Itarte
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J N Dholakia, A J Wahba

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Citations

Aug 31, 2001·Progress in Neurobiology·C A Grimes, R S Jope
Jan 23, 1998·The International Journal of Biochemistry & Cell Biology·B L Webb, C G Proud
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·L S JeffersonS R Kimball
Mar 5, 2003·Journal of Cell Science·Bradley W Doble, James R Woodgett
Sep 1, 2015·Archives of Biochemistry and Biophysics·Medchalmi Swetha, Kolluru Va Ramaiah
Apr 16, 1998·The Journal of Biological Chemistry·M KleijnA A Thomas
Oct 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Thomas C VaryScot R Kimball
Sep 5, 2001·The Journal of Nutrition·L S Jefferson, S R Kimball

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