Modulation of radiosensitivity in Chinese hamster lung fibroblasts by cisplatin

Canadian Journal of Physiology and Pharmacology
J M Akudugu, J P Slabbert

Abstract

The effects of cisplatin exposure time, concentration, and irradiation sequence on the sensitivity of Chinese hamster lung fibroblasts (V79) to gamma-ray exposure were examined. Based on clonogenic cell survival, the cisplatin concentrations corresponding to 50% cell survival (EC(50)) for exposure times of 1 h to 7 days followed a 2-phase exponential decay and ranged from 28.26 +/- 3.32 to 1.53 +/- 0.24 micromol/L, respectively. When cells were treated at EC(50) for exposures of less than 4 h and irradiated immediately, cisplatin inhibited the effect of radiation. Exposures of 4-6 h did not affect radiosensitivity. For exposures of 8-12 h, radiosensitization was observed, which disappeared at 14 h and reappeared for much longer cisplatin treatments. At the lowest achievable EC(50) (1.53 micromol/L), radiosensitization was observed if irradiation was delayed for 1-8 h. This enhancement in radiosensitivity disappeared for irradiation delays of 10-12 h, but reappeared when irradiation was delayed for 14-18 h. These data demonstrate that the mode of interaction between cisplatin and gamma-irradiation depends on the concentration and exposure time of cisplatin, as well as on the timing of irradiation after cisplatin administration. ...Continue Reading

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Citations

May 19, 2009·Canadian Journal of Physiology and Pharmacology·B B Mokaleng, J M Akudugu
Feb 1, 2013·International Journal of Radiation Biology·John M AkuduguLothar J F Böhm
Jan 22, 2010·Molecular Ecology·Etienne SlabbertKarin Jacobs
Dec 29, 2010·Journal of Radiation Research·Kazue MizunoMitsuru Uesaka
Mar 23, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·John M AkuduguRoger W Howell

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