PMID: 9555575Apr 29, 1998Paper

Modulation of rat lung Na+,K(+)-ATPase gene expression by hyperoxia

Experimental Lung Research
C R JohnsonR M Jackson

Abstract

Rats exposed to 85% O2 for 5-7 days develop tolerance to otherwise lethal hyperoxia (100% O2). The rate of alveolar fluid clearance increases during adaptation to hyperoxia, due in part to increased alveolar epithelial sodium channel activity. In these studies, we have investigated molecular mechanisms leading to increased lung Na+,K(+)-ATPase activity in hyperoxia. We exposed adult rats to 85% O2 (sublethal hyperoxia) for 7 days, followed by 2, 3, or 4 days in 100% O2. Steady-state levels of the Na+,K(+)-ATPase alpha 1 and beta 1 subunit mRNAs increased in whole lung tissue during hyperoxia exposures. Stability of the Na+,K(+)-ATPase alpha 1 and beta 1 subunit mRNA messages in whole lung RNA did not change significantly. Thus, lung Na+,K(+)-ATPase gene expression in sublethal hyperoxia appears to be regulated in part at the transcriptional level. Alveolar epithelial type II (ATII) cell Na+,K(+)-ATPase alpha 1 and beta 1 subunit proteins, measured by quantitative immunofluorescence, increased significantly after sublethal hyperoxia and 100% O2 exposures. Increases in lung fluid clearance after sublethal hyperoxia are associated with increased ATII cell Na+,K(+)-ATPase protein and whole lung Na+,K(+)-ATPase mRNA expression, whic...Continue Reading

References

Jan 1, 1991·Annual Review of Physiology·J D HorisbergerB C Rossier
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Citations

Jan 2, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christine Clerici, Carole Planès
Jan 21, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ulrich H ThomeSadis Matalon
Jun 11, 1999·The American Journal of Physiology·Z BorokR L Lubman

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