Modulation of rat vaginal blood flow and estrogen receptor by estradiol

The Journal of Urology
Soo Woong KimAbdulmaged M Traish

Abstract

The effects of subphysiological and physiological levels of estradiol on vaginal blood flow and estrogen receptor were investigated. Intact or ovariectomized female Sprague-Dawley rats were used. Two weeks after surgery rats were infused with vehicle (polyethyleneglycol), or estradiol at subphysiological (5 microg daily) or physiological (15 microg daily) concentrations for 14 days using osmotic pumps. Changes in vaginal blood flow elicited by pelvic nerve stimulation were assessed by laser Doppler flowmetry. Total levels of functional estrogen receptor were determined by radioligand binding and Western blot analyses were used to assess estrogen receptor (ER) alpha protein. Mean plasma estradiol concentration +/- SEM decreased by 63% in the vehicle group (intact 36.5 +/- 10.3 pg/ml). The subphysiological and physiological estradiol groups had plasma levels that were 55% and 83% of the intact group, respectively. Uterine and vaginal wet weight, and vaginal blood flow were significantly decreased in the vehicle group and normalized by physiological levels of estradiol. However, vaginal blood flow was significantly greater in the subphysiological estradiol group compared to intact animals. Specific binding of [H]estradiol in vagin...Continue Reading

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Feb 10, 2004·The Journal of Urology·Soo Woong KimAbdulmaged M Traish

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Citations

Sep 7, 2012·The British Journal of Nutrition·Li-Chan YangWen-Chuan Lin
Nov 28, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Hui-Ya HoWen-Chuan Lin
Jun 15, 2011·Reproductive Sciences·Zhaohui Liao, Peter G Smith
Sep 15, 2006·BMC Women's Health·Noel N KimAbdulmaged M Traish
Feb 3, 2016·Climacteric : the Journal of the International Menopause Society·T TzurA Y Weintraub
Jan 14, 2009·The Journal of Sexual Medicine·Biljana MusickiArthur L Burnett
May 15, 2007·The Journal of Sexual Medicine·Abdulmaged M TraishNoel N Kim
Aug 31, 2007·The Journal of Sexual Medicine·Abdulmaged TraishUNKNOWN Testosterone Therapy in Women Study Group
Mar 18, 2010·The Journal of Sexual Medicine·Biljana MusickiArthur L Burnett
Jan 21, 2006·The Journal of Sexual Medicine·Annamaria GiraldiIrwin Goldstein
Jul 16, 2010·The Journal of Sexual Medicine·Abdulmaged M TraishNoel N Kim
May 12, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Tulay CushmanAbdulmaged M Traish
Jun 10, 2011·Alcoholism, Clinical and Experimental Research·Courtney S Vetter-O'Hagen, Linda P Spear
Dec 14, 2011·Journal of Ethnopharmacology·Yu-Jen KoWen-Chuan Lin
May 15, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Mikko UnkilaRisto Lammintausta
Dec 30, 2014·Biology of Reproduction·T Ignacio MontoyaR Ann Word
Jul 14, 2006·Menopause : the Journal of the North American Menopause Society·Xiao-Ning WangDavid D Thompson
May 17, 2007·The Journal of Pharmacology and Experimental Therapeutics·Marcel GehlhausRalf P Meyer
May 28, 2020·International Journal of Molecular Sciences·Elena S GardiniUlrike Ehlert

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