PMID: 8955297Dec 1, 1996Paper

Modulation of recombination and DNA repair by the RecG and PriA helicases of Escherichia coli K-12

Journal of Bacteriology
A A Al-DeibR G Lloyd

Abstract

The RecG protein of Escherichia coli is a structure-specific DNA helicase that targets strand exchange intermediates in genetic recombination and drives their branch migration along the DNA. Strains carrying null mutations in recG show reduced recombination and DNA repair. Suppressors of this phenotype, called srgA, were located close to metB and shown to be alleles of priA. Suppression depends on the RecA, RecBCD, RecF, RuvAB, and RuvC recombination proteins. Nine srgA mutations were sequenced and shown to specify mutant PriA proteins with single amino acid substitutions located in or close to one of the conserved helicase motifs. The mutant proteins retain the ability to catalyze primosome assembly, as judged by the viability of recG srgA and srgA strains and their ability to support replication of plasmids based on the ColE1 replicon. Multicopy priA+ plasmids increase substantially the recombination- and repair-deficient phenotype of recG strains and confer similar phenotypes on recG srgA double mutants but not on ruvAB or wild-type strains. The multicopy effect is eliminated by K230R, C446G, and C477G substitutions in PriA. It is concluded that the 3'-5' DNA helicase/translocase activity of PriA inhibits recombination and t...Continue Reading

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Citations

Oct 10, 1997·Nucleic Acids Research·A A MahdiR G Lloyd
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·R Friedman-Ohana, A Cohen
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M M Cox
Sep 5, 2001·Molecular Microbiology·S J SandlerR U Johansen
Mar 31, 2004·Molecular Microbiology·Tom R MeddowsRobert G Lloyd
Dec 16, 2005·The Journal of Biological Chemistry·Taku Tanaka, Hisao Masai
Jan 10, 2017·Frontiers in Microbiology·Martin VestergaardHanne Ingmer
Feb 6, 2017·FEBS Letters·Benura Azeroglu, David R F Leach
Dec 5, 2017·Nucleic Acids Research·Tricia A WindgassenJames L Keck
Feb 13, 2003·Molecular Microbiology·Razieh P Jaktaji, Robert G Lloyd
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·A Kuzminov
Apr 7, 2004·Molecular Microbiology·Gianfranco GromponeBénédicte Michel
Nov 19, 2008·Critical Reviews in Biochemistry and Molecular Biology·Nicole S Persky, Susan T Lovett
Jul 27, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hisao MasaiDaisuke Kohda
Jun 23, 2009·Molecular Microbiology·Christian J RudolphRobert G Lloyd
Jan 15, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Cong YuPiero R Bianco
Feb 13, 2016·PLoS Genetics·Benura AzerogluDavid R F Leach
Apr 4, 2016·Current Genetics·Robert G Lloyd, Christian J Rudolph
May 30, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M M Cox
Jun 8, 2010·Nucleic Acids Research·Geoffrey S BriggsRobert G Lloyd
Aug 8, 2014·PLoS Genetics·Julia S P Mawer, David R F Leach
Mar 23, 2017·Journal of Bacteriology·Bénédicte Michel, Steven J Sandler
Aug 24, 1999·Nucleic Acids Research·P McGlynn, R G Lloyd
Apr 7, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Geoffrey S BriggsRobert G Lloyd
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Ryan C Heller, Kenneth J Marians
Feb 8, 2005·The Journal of Biological Chemistry·Geoffrey S BriggsRobert G Lloyd
Sep 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tricia A WindgassenJames L Keck
Jul 25, 1998·The Journal of Biological Chemistry·M C Whitby, R G Lloyd
Jun 15, 2018·Microbiology and Molecular Biology Reviews : MMBR·Bénédicte MichelDavid R F Leach
Oct 29, 2000·Journal of Bacteriology·M J Lombardo, S M Rosenberg
Aug 28, 1999·Journal of Bacteriology·A R PoteeteK C Murphy
Apr 15, 2020·FEMS Microbiology Reviews·Anurag Kumar SinhaDavid R F Leach

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