Modulation of RNA polymerase core functions by basal transcription factor TFB/TFIIB.

Biochemical Society Symposium
Finn WernerRobert O J Weinzierl

Abstract

The archaeal basal transcriptional machinery consists of TBP (TATA-binding protein), TFB (transcription factor B; a homologue of eukaryotic TFIIB) and an RNA polymerase that is structurally very similar to eukaryotic RNA polymerase II. This constellation of factors is sufficient to assemble specifically on a TATA box-containing promoter and to initiate transcription at a specific start site. We have used this system to study the functional interaction between basal transcription factors and RNA polymerase, with special emphasis on the post-recruitment function of TFB. A bioinformatics analysis of the B-finger of archaeal TFB and eukaryotic TFIIB reveals that this structure undergoes rapid and apparently systematic evolution in archaeal and eukaryotic evolutionary domains. We provide a detailed analysis of these changes and discuss their possible functional implications.

Citations

Jun 19, 2021·Frontiers in Molecular Biosciences·Sven Nottebaum, Robert O J Weinzierl

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
O LittlefieldP B Sigler
© 2022 Meta ULC. All rights reserved