Nov 4, 2018

Modulation of rpoS fitness by loss of cpdA activity during stationary-phase in Escherichia coli

BioRxiv : the Preprint Server for Biology
Savita Chib, Aswin Sai Narain Seshasayee

Abstract

Experimental evolution of Escherichia coli in one month long stationary-phase in lysogeny broth batch cultures repeatedly selected mutations in the genes for the stationary-phase sigma factor RpoS and the cAMP phosphodiesterase CpdA. The founder strain carried a previously identified allele of rpoS, referred to as rpoS819, a partially functional variant that confers growth advantage in stationary-phase (GASP). The 46 base duplication at the 3′ end of rpoS819 produces a longer protein present at very low levels compared to wild type RpoS. A new rpoS variant rpoS92, carrying a re-duplication of the original duplication in rpoS819, arose during the first week of our evolution experiment. In rpoS92, an in-frame stop codon truncated RpoS819 creating a shorter RpoS92 whose levels are restored to that of wild type RpoS. Transcription profiling of rpoS92 indicated a shift in gene-expression to that of wild-type rpoS, reversing some of the expression trends of rpoS820. Δ3cpdA, carrying an in-frame three base deletion, had arisen late in our evolution experiment. It is a loss of function mutation, which elevates cAMP levels. Using mixed culture competition experiments, we demonstrate that rpoS92 confers GASP, whereas Δ3cpdA confers relat...Continue Reading

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Mentioned in this Paper

Lysogeny
Genes
Transcription, Genetic
Codon, Terminator
Gene Deletion Abnormality
Gene Deletion
Gasping for Breath
Cyclic AMP
Gene Mutation
Gene Expression

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