Modulation of Schlemm's canal endothelial cell stiffness via latrunculin loaded block copolymer micelles

Journal of Biomedical Materials Research. Part a
Trevor StackEvan Alexander Scott

Abstract

Increased stiffness of Schlemm's canal endothelial cells (SC cells) is a major contributing factor to the increased pressure characteristic of primary open-angle glaucoma. New treatments for glaucoma are being developed using actin depolymerizers and rho kinase inhibitors to address this increased stiffness. However, these agents have off-target effects and are not as potent as had been hoped. We have developed a micellar nanocarrier assembled from poly(ethylene glycol)-bl-poly(propylene sulfide) copolymers capable of encapsulating latrunculin A (Lat A) with the goal of modulating SC cell stiffness. Lat A-loaded nanocarriers were similar in size and morphology to unloaded poly (ethylene glycol)-bl-poly(propylene sulfide) (PEG-bl-PPS) micelles, loaded Lat A at 62% encapsulation efficiency, and retained loaded Lat A for at least 22 days. The continued functional activity of Lat A following encapsulation within micelles was verified in murine macrophages, which are known to display decreased endocytosis in response to Lat A-dependent cytoskeletal disruption. Endocytic inhibition remained unchanged when comparing equal concentrations of micelle-loaded versus free form Lat A. Uptake of Lat A-loaded micelles by human SC cells was ver...Continue Reading

References

Mar 14, 2000·Experimental Eye Research·J A PetersonP L Kaufman
Jun 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Huajian GaoLambert B Freund
Feb 21, 2006·The British Journal of Ophthalmology·H A Quigley, A T Broman
Mar 25, 2006·Immunology·Christopher C Norbury
Mar 16, 2007·Bioconjugate Chemistry·Tatiana Segura, Jeffrey A Hubbell
Jun 16, 2009·Nature Materials·Andre E NelMike Thompson
Aug 29, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Simona CerritelliJeffrey A Hubbell
Jan 12, 2012·Investigative Ophthalmology & Visual Science·Paul Russell, Mark Johnson
Jan 17, 2012·Investigative Ophthalmology & Visual Science·Sara M ThomasyPaul Russell
Aug 10, 2012·Journal of Virology·Jeremy S RossmanRobert A Lamb
Aug 21, 2012·ACS Nano·Andreas E VasdekisJeffrey A Hubbell
Oct 16, 2012·Investigative Ophthalmology & Visual Science·Paul Russell, Mark Johnson
Feb 14, 2013·ACS Nano·Heather HerdClaus-Michael Lehr
Mar 30, 2013·Cellular Logistics·Dipannita Dutta, Julie G Donaldson
Jul 23, 2013·Biophysical Journal·R Vargas-PintoM Johnson
Dec 7, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Casey C Kopczynski, David L Epstein
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Darryl R OverbyMark Johnson
Sep 23, 2014·Translational Vision Science & Technology·Carol A RasmussenJason L Vittitow
Oct 20, 2015·Journal of Chemical Information and Modeling·Teague Sterling, John J Irwin
May 3, 2016·Regenerative Engineering and Translational Medicine·Sean AllenEvan Scott
Feb 23, 2017·Annual Review of Biomedical Engineering·Evan A ScottPunn Augsornworawat
Mar 30, 2017·Advances in Therapy·Vanessa Andrés-GuerreroAnastasios Georgios Konstas
Jul 25, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sean AllenEvan Scott

❮ Previous
Next ❯

Citations

Aug 11, 2018·Journal of Cellular Physiology·Jing-Yi ZhuJian Ye
Jul 31, 2020·Expert Opinion on Drug Metabolism & Toxicology·Vrinda GoteDhananjay Pal
Aug 17, 2020·Progress in Retinal and Eye Research·Ted S AcottMary J Kelley
Apr 23, 2021·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Trevor StackEvan Scott
Jul 8, 2021·ACS Applied Materials & Interfaces·Michael P VincentEvan A Scott
Aug 12, 2019·ACS Biomaterials Science & Engineering·Carlos Matellan, Armando E Del Río Hernández

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.