Modulation of sensitivity of blood forms of Trypanosoma cruzi to antibody-mediated, complement-dependent lysis.

Infection and Immunity
F Kierszenbaum, Marcel I Ramirez

Abstract

The numerous reports on lysis of blood (trypomastigote) forms of Trypanosoma cruzi by specific antibodies plus complement have systematically shown that a certain proportion of parasites survives. However, it is not known whether the insensitive organisms represent a subpopulation (or clones) or a certain developmental phase of otherwise morphologically identical parasites. In this work, we established that partial lysis was not due to the use of insufficient amounts of lytic reagents. Thus, supernatants of lytic reaction mixtures killed the same proportion of T. cruzi as previously unused reagents. Moreover, in parallel tests in which the trypomastigote concentration was up to four times greater than that used in standard lysis tests, the percentages of lysis were comparable. Incubation periods as long as 4 h did not increase the extent of lysis beyond the value observed after only 1 h, indicating that the routinely used 1-h incubation was appropriate. The extent of lysis was not increased by additional amounts of antibody, complement, or both. Instead, trypomastigotes surviving immune lysis, washed, and incubated with fresh diluent for 45 to 120 min before being used in new lysis tests did manifest additional sensitivity to i...Continue Reading

References

Jan 1, 1988·Parasitology Research·R L CardoniE L Segura
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M T RimoldiK Joiner
Nov 1, 1972·The Journal of protozoology·F Kierszenbaum, L E Saavedra
Jan 1, 1980·Advances in Parasitology·Z Brener
Nov 1, 1983·The American Journal of Tropical Medicine and Hygiene·F Kierszenbaum, M F Lima

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Citations

Mar 27, 2003·Vector Borne and Zoonotic Diseases·Miwako Takano-LeeMiercio E A Pereira
Dec 21, 2020·Immunobiology·Ana K A SoaresVirginia M B Lorena
Oct 11, 2003·Molecular and Biochemical Parasitology·Patricia BerasainFernando Goñi

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