Modulation of skeletal muscle protein synthesis by amino acids and insulin during sepsis

Metabolism: Clinical and Experimental
C JurasinskiThomas C Vary

Abstract

Effects of different concentrations of insulin and amino acids on protein synthesis in skeletal muscle of young, fed septic rats were determined in the perfused rat hindlimb. Rates of protein synthesis in gastrocnemius were measured by incorporation of [3H]-phenylalanine into protein. Perfusion of hindlimb muscles from young, fed control rats with medium containing either insulin and a complete mixture of amino acids at plasma concentration (1x) or a mixture of amino acids at 10-fold (10x) plasma concentration resulted in an approximately twofold stimulation of the rate of protein synthesis. The effect of amino acids on protein synthesis was partly accounted for by elevated concentrations of branched-chain amino acids ([BCAA] leucine, isoleucine, and valine). In young, fed septic rats, the rate of protein synthesis in muscle perfused with buffer containing the normal concentration of amino acids was reduced 40% as compared with control levels (P < .05). In contrast to controls, addition of insulin (1,000 microU/mL) did not augment protein synthesis in muscle from young, fed septic rats perfused with the complete mixture of amino acids. Addition of insulin 10,000 microU/mL stimulated protein synthesis approximately 80% in gastro...Continue Reading

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Citations

Aug 11, 2000·Journal of Gastroenterology and Hepatology·C PlatellJ C Hall
Jun 1, 2004·Nutrition Research Reviews·Thomas C Vary, Christopher J Lynch
May 21, 2010·American Journal of Physiology. Endocrinology and Metabolism·Alexander P TuckowLeonard S Jefferson
Apr 15, 2010·American Journal of Physiology. Endocrinology and Metabolism·Charles H LangThomas C Vary
Jun 18, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Charles H LangThomas C Vary
Apr 15, 2010·Cancer Research·Byoung Kwon YooDevanand Sarkar
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