Modulation of store-operated calcium entry and nascent adhesion by p21-activated kinase 1

Experimental & Molecular Medicine
In-Sook JeonJoong-Kook Choi

Abstract

Calcium mobilization is necessary for cell movement during embryonic development, lymphocyte synapse formation, wound healing, and cancer cell metastasis. Depletion of calcium in the lumen of the endoplasmic reticulum using inositol triphosphate (IP3) or thapsigargin (TG) is known to induce oligomerization and cytoskeleton-mediated translocation of stromal interaction molecule 1 (STIM1) to the plasma membrane, where it interacts with the calcium release-activated calcium channel Orai1 to mediate calcium influx; this process is referred to as store-operated calcium entry (SOCE). Furthermore, aberrant STIM1 or SOCE regulation is associated with cancer cell motility and metastasis. The p21-activated kinases (PAKs), which are downstream effectors of GTPases, reportedly regulate cytoskeletal organization, protrusive activity, and cell migration. Although cytoskeletal remodeling apparently contributes to calcium mobilization via SOCE, and vice versa, the mechanisms by which they regulate each other remain unclear. In this study, we aimed to characterize whether PAK1 modulates calcium mobilization and STIM1 localization. Our data demonstrate that PAK1 interacts with STIM1 in vitro and that this interaction was enhanced by treatment wi...Continue Reading

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Citations

Aug 23, 2020·International Journal of Molecular Sciences·Jarosław NuszkiewiczKarolina Szewczyk-Golec
Jun 6, 2020·Archives of Pharmacal Research·In-Sook JeonJoong-Kook Choi
Sep 2, 2020·Cell Communication and Signaling : CCS·Jizheng GuoBing Shen
Dec 20, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fangfang XuBing Shen

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Methods Mentioned

BETA
transfection
confocal microscopy
pull-down
pull down
immunoprecipitation
GTPases

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