Modulation of TGFβ(2) and dopamine by PKC in retinal Müller cells of guinea pig myopic eye.

International Journal of Ophthalmology
Jun-Feng MaoQian Xiang

Abstract

To investigate the effect of protein kinase C (PKC) on transforming growth factor-β(2) (TGFβ(2)) and dopamine in retinal Müller cells of guinea pig myopic eye. Myopia was induced by translucent goggles in guinea pig, whose retinal Müller cells were cultured using the enzyme-digesting method. Retinal Müller cells were divided into 5 groups: normal control, myopia, myopia plus GF109203X, myopia plus PMA, myopia plus DMSO. PKC activities were detected by the non-radioactive methods. TGFβ(2) and tyrosine hydroxylase (TH) proteins were analyzed by Western Blotting in retinal Müller cells. Dopamine was determined by the high-performance liquid chromatography-electrochemical detection in suspensions. After 14 days deprived, the occluded eyes became myopic with ocular axle elongating. Müller cells of guinea pigs were obtained using enzyme digestion. Compared with normal control group, the increase in PKC activity and the up-regulation in TGFβ(2) expression were found in retinal Müller cells of myopic eyes, with the decrease of TH and dopamine content (P<0.05). After PKC activated by PMA, TGFβ(2) and TH content were up-regulated with the increase of dopamine content (P<0.05). While the PKC activities was inhibited by GF109203X, proteins...Continue Reading

Citations

Dec 5, 2019·Scientific Reports·Kate ThomsonRegan Ashby
Aug 9, 2020·Scientific Reports·Kate ThomsonRegan Ashby

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