PMID: 22580534May 15, 2012Paper

Modulation of Th1/Th2 cytokine production by selective and nonselective phosphodiesterase inhibitors administered to mice

Pharmacological Reports : PR
Marianna SzczypkaBożena Obmińska-Mrukowicz

Abstract

Phosphodiesterase (PDE) inhibitors can modulate the functions of immune cells, including T lymphocytes, due to increased intracellular levels of cyclic nucleotides. The drugs (aminophylline, milrinone and sildenafil) were administered once or five times at 24 h intervals at the following doses: 20 mg/kg, i.m., 1 mg/kg, i.m. and 1 mg/kg, p.o., respectively. Th1 and Th2 cytokine levels (IL-2, IFN-γ, IL-4, IL-5, TNF) were determined 12, 24 or 72 h after the last administration of the drugs. A commercial BD™ Cytometric Bead Array Mouse Th1/Th2 Cytokine Kit (CBA) was used to determine the levels of Th1/Th2 cytokines in the serum. Neither of the PDE inhibitors under investigation administered once changed IFN-γ, TNF and IL-4 production. A single dose of aminophylline decreased the production of IL-2 (after 12 h). A single dose of milrinone did not affect Th1/Th2 cytokine secretion. Sildenafil administered once decreased the production of IL-2 (after 72 h). A temporary enhancement in the level of IL-5 was observed 12 h after a single dose of sildenafil. No changes in Th1 and Th2 cytokine production were observed after five doses of PDE inhibitors under investigation. These results indicate that nonstimulated lymphocytes Th1 and Th2 ex...Continue Reading

References

Dec 1, 1987·The Journal of Experimental Medicine·E A Kurt-JonesA K Abbas
Aug 1, 1993·Immunology Letters·K RieneckK Bendtzen
Jul 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H TenorJ K Shute
Feb 26, 1998·American Journal of Respiratory and Critical Care Medicine·T J Torphy
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B BielekovaR Martin
Mar 1, 2002·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Ching-Chi LinAnn Chen
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Radhakrishnan SureshSatyajit Rath
Dec 21, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Revital ArichaSara Fuchs
Feb 7, 2006·European Journal of Pharmacology·Kian Fan Chung
Mar 5, 2008·Journal of Occupational Medicine and Toxicology·Gaetano CaramoriAlberto Papi
Jan 1, 2008·International Journal of Chronic Obstructive Pulmonary Disease·David M G Halpin

❮ Previous
Next ❯

Citations

Mar 1, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·X J YaoS Ying
Oct 26, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yanfei BinZhi-Yi He
Feb 18, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Beata Kaleta, Agnieszka Boguska
Jan 10, 2020·Journal of Immunology Research·Michał ZychRadoslaw Zagozdzon
Apr 1, 2015·Journal of Microscopy and Ultrastructure·Mohammed A Afifi, Mohammed W Al-Rabia
Mar 21, 2017·Journal of Immunology Research·Monika Kniotek, Agnieszka Boguska
Aug 18, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·YuFei HeYing Xie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.