Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3

Journal of Cellular Physiology
C L Hardy, J J Minguell

Abstract

The integrins are a class of adhesion molecules which have been implicated in the homing of hemopoietic stem cells and in their restriction within the bone marrow. Integrins function as mediators of cell-extracellular matrix (ECM) interactions amd also of cell-cell interactions. They are unique membrane receptors which are capable of activation, change in affinity, and change in expression. Because of their broad potential for modulation we examined the effect of a cytokine growth factor which is present constitutively in the marrow, interleukin 3 (IL3), on integrin-mediated adherence of hemopoietic progenitor cells to the matrix component fibronectin (FN). The multipotential murine cell line B6Sut and the committed granulocyte progenitor cell line FDCP-1 were used. Both of these cell lines have been shown to bind to FN-coated dishes and to dishes coated with the 120 kDa and 40 kDa chymotryptic fragments of FN. It was found that after a brief withdrawal of IL3 the cells lost 80% adherence to the 120 kDa FN fragment containing the RGD cell binding site. This loss of binding was not related to a loss of viability, appeared unrelated to the growth/survival activity of IL3, and was quickly reversible by readdition of the growth fac...Continue Reading

References

Jul 1, 1992·The Journal of Clinical Investigation·M W LongR Hoffman
Aug 1, 1992·The Journal of Clinical Investigation·J TeixidóP Anklesaria
Aug 2, 1990·Nature·T A Springer
Apr 1, 1990·Immunology Today·D J McConkeyM Jondal
Jan 1, 1990·Annual Review of Immunology·K Dorshkind
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P AnklesariaJ Massagué
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S Aizawa, M Tavassoli
Mar 1, 1989·The Journal of Clinical Investigation·T MatsuokaM Tavassoli
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P AnklesariaJ S Greenberger
Jun 1, 1987·The Journal of Clinical Investigation·C A Sieff
Jun 5, 1987·Science·S C Clark, R Kamen
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Aizawa, M Tavassoli
Jan 1, 1987·Journal of Cellular Physiology·M Y GordonM F Greaves
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J S GreenbergerR J Eckner
Oct 1, 1980·The Journal of Experimental Medicine·T M DexterD Metcalf
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
May 1, 1994·In Vitro Cellular & Developmental Biology. Animal·E WeberG Sacchi
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T Papayannopoulou, B Nakamoto
Sep 19, 1993·Biochimica Et Biophysica Acta·J J MinguellM Tavassoli
Mar 1, 1993·Immunology Today·J J Cohen

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