Modulation of the c-Jun N-terminal kinase activity in the embryonic heart in response to anoxia-reoxygenation: involvement of the Ca2+ and mitoKATP channels.

Molecular and Cellular Biochemistry
Alexandre SarreEric Raddatz

Abstract

Whether the response of the fetal heart to ischemia-reperfusion is associated with activation of the c-Jun N-terminal kinase (JNK) pathway is not known. In contrast, involvement of the sarcolemmal L-type Ca2+ channel (LCC) and the mitochondrial KATP (mitoKATP) channel has been established. This work aimed at investigating the profile of JNK activity during anoxia-reoxygenation and its modulation by LCC and mitoK(ATP) channel. Hearts isolated from 4-day-old chick embryos were submitted to anoxia (30 min) and reoxygenation (60 min). Using the kinase assay method, the profile of JNK activity in the ventricle was determined every 10 min throughout anoxia-reoxygenation. Effects on JNK activity of the LCC blocker verapamil (10 nM), the mitoK(ATP) channel opener diazoxide (50 microM) and the blocker 5-hydroxydecanoate (5-HD, 500 microM), the mitochondrial Ca2+ uniporter (MCU) inhibitor Ru360 (10 microM), and the antioxidant N-(2-mercaptopropionyl) glycine (MPG, 1 mM) were determined. In untreated hearts, JNK activity was increased by 40% during anoxia and peaked fivefold relative to basal level after 30-40 min reoxygenation. This peak value was reduced by half by diazoxide and was tripled by 5-HD. Furthermore, the 5-HD-mediated stimul...Continue Reading

References

Feb 1, 1991·Proceedings of the Society for Experimental Biology and Medicine·R G Allen
Jan 5, 1996·Biochemical and Biophysical Research Communications·R J Knight, D B Buxton
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·R M LebovitzM M Matzuk
Feb 25, 1998·Journal of Cardiovascular Pharmacology·D TenthoreyE Raddatz
Jun 18, 1999·Journal of Molecular and Cellular Cardiology·T OmuraJ Yoshikawa
Mar 3, 2001·Pediatric Research·R RomanoE Raddatz
Oct 31, 2001·Cardiovascular Research·R SchulzG Heusch
Jul 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David SedmeraEric Raddatz
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Gilles LebuffeTerry L Vanden Hoek
May 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·A RosaE Raddatz
Apr 24, 2004·BMJ : British Medical Journal·Sailesh Kumar, Anna O'Brien
Jul 1, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher J DoughertyKeith A Webster
Oct 28, 2004·Biochemical Society Transactions·C WiltshireG H W May
Nov 20, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Alexandre SarreEric Raddatz
Dec 14, 2004·Free Radical Biology & Medicine·Craig Horbinski, Charleen T Chu
Apr 6, 2005·Journal of Molecular and Cellular Cardiology·Jie RenAnthony J Muslin
May 26, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Christopher D AndersonRavi S Chari
Sep 22, 2005·Physiology·Brian O'RourkeMiguel A Aon
Dec 6, 2005·Clinics in Perinatology·Janette F Strasburger
Jan 31, 2006·Physiology·Abigail L FowdenAlison J Forhead
Jun 13, 2006·Journal of Molecular and Cellular Cardiology·Marcus C SchaubMichael Zaugg
Oct 4, 2006·Journal of Cardiovascular Electrophysiology·Alexandre SarreEric Raddatz
Feb 9, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Marina ComelliIrene Mavelli

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Citations

Mar 23, 2010·Molecular and Cellular Biochemistry·Stephany GardierEric Raddatz
Sep 29, 2009·Molecular and Cellular Biochemistry·Vani NilakantanChristopher P Johnson
Nov 5, 2014·Acta Physiologica·D SedmeraE Raddatz

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