Abstract
We investigated how varying the composition of cell culture formulations and growing cancer cells at different densities might affect tumor cell genotype. Specifically, we compared gene expression profiles generated by human MDA‑MB‑231 breast cancer cells cultured in different media [minimum essential medium (MEM), Dulbecco's modified Eagle's medium (DMEM), or Roswell Park Memorial Institute (RPMI)‑1640 medium] containing different concentrations of fetal bovine serum (FBS) or different sera (equine or bovine) that were grown at different cell densities. More than 2,000 genes were differentially modulated by at least a 2‑fold difference when MDA‑MB‑231 cancer cells were 90% confluent and compared with cultures that were 50% confluent. Altering the concentration of serum produced an even more pronounced effect on MDA‑MB‑231 cancer cell gene expression in that 2,981 genes were differentially expressed in a comparison between cells cultured in 0.1% FBS and same cell density cultures that were maintained in 10% FBS. A comparison between MDA‑MB‑231 cancer cells that were 90% confluent in MEM, DMEM, or RPMI‑1640 media, all containing 10% FBS, resulted in 8,925 differentially expressed genes. Moreover, one‑quarter (25.6%) of genes fro...Continue Reading
References
May 1, 1996·Nature Medicine·B J DrukerN B Lydon
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Jan 5, 2000·The Journal of Clinical Investigation·B J Druker, N B Lydon
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 18, 2002·Nature·Helen DaviesP Andrew Futreal
Aug 3, 2005·PLoS Medicine·John P A Ioannidis
Apr 28, 2007·Science·Jeffrey A EngelmanPasi A Jänne
Nov 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David J J Waugh, Catherine Wilson
Feb 6, 2009·Journal of the American Society of Nephrology : JASN·Min-A YuDuk-Hee Kang
Oct 8, 2009·Molecular Cancer Research : MCR·Tae Hyong KimJun Chung
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Jan 19, 2010·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Adi F GazdarJohn D Minna
May 8, 2010·Nature Reviews. Cancer·UNKNOWN American Type Culture Collection Standards Development Organization Workgroup ASN-0002
Jul 9, 2010·In Vitro Cellular & Developmental Biology. Animal·Rita BarallonLiz Kerrigan
Aug 4, 2010·Journal of the National Cancer Institute·Adi F GazdarJohn D Minna
Mar 3, 2011·International Journal of Cancer. Journal International Du Cancer·Robert R Langley, Isaiah J Fidler
Mar 11, 2011·Neoplasia : an International Journal for Oncology Research·Sun-Jin KimIsaiah J Fidler
Mar 26, 2011·The Journal of Clinical Investigation·Tina CasconeJohn V Heymach
Sep 6, 2011·Nature Reviews. Drug Discovery·Florian PrinzKhusru Asadullah
Mar 31, 2012·Nature·C Glenn Begley, Lee M Ellis
May 5, 2012·Seminars in Cancer Biology·Christina Scheel, Robert A Weinberg
Sep 18, 2013·Breast Cancer Research : BCR·Jagdeep K SinghRobert B Clarke
Oct 8, 2013·Cell Death and Differentiation·P-P LiuP Huang
Nov 10, 2013·Nature Medicine·Wai Leong Tam, Robert A Weinberg
Nov 26, 2013·Nature·Mina Bissell
Jan 23, 2014·Diabetes Research and Clinical Practice·Lin TangFengmei Chen
Citations
Sep 15, 2017·PloS One·Markus FehrholzSteffen Kunzmann
Oct 19, 2016·Cell Cycle·Audrey BertheSabine Mazerbourg
Dec 31, 2016·Glycoconjugate Journal·Qingsong XuLinsheng Song
Apr 8, 2017·Scientific Reports·Marta E PolakMichael R Ardern-Jones
May 17, 2019·Antioxidants·Lisa Arodin SeleniusMikael Björnstedt
Oct 30, 2020·Scientific Reports·Diane Frances LeeMark Andrew Chambers