Modulation of the dual-faced effects of miR-141 with chitosan/miR-141 nanoplexes in breast cancer cells

The Journal of Gene Medicine
Kubra KabanJulide Akbuga

Abstract

miR-141, known as a tumor suppressive microRNA, is downregulated in breast cancer. However, recent contrasting studies report that it also acts as oncogene when it is upregulated. The present study aimed to investigate whether miR-141 is a tumor suppressor or oncogenic when it reaches normal levels in chitosan/miR-141 nanoplexes. Chitosan nanoplexes were prepared using simple complexation method. Nanoplexes were characterized by a gel retardation assay and zeta potential and particle size measurements. To determine the expression level of miR-141, a quantitative real-time polymerase chain reaction was performed. The effects of miR-141 mimics were investigated with respect to angiogenesis by vascular endothelial growth factor (VEGF), epithelial-mesenchymal transition (EMT) by E-cadherin, metastasis by Igfbp-4 and Tinagl1 enzyme-linked immunosorbent assays, invasion by an invasion chamber, and apoptosis by Annexin V. The miR-141 expression levels of MDA-MB-231 and MDA-MB-435 cells by administration of chitosan/mimic miR-141 nanoplexes reached endogenous miR-141 levels of a non-tumorigenic epithelial breast cell line, MCF-10A. According to our results, metastasis, VEGF, EMT and invasion in breast cancer cells were diminished, wher...Continue Reading

References

Sep 1, 2006·Genome Biology·Ola Snøve, John J Rossi
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Apr 28, 2007·Advanced Drug Delivery Reviews·Christine C Esau, Brett P Monia
Jul 28, 2009·Annual Review of Medicine·Ramiro GarzonCarlo M Croce
Sep 30, 2009·PloS One·Derek M DykxhoornJudy Lieberman
Mar 30, 2010·RNA·Sophie BailMegerditch Kiledjian
Jul 2, 2010·Cancer Biology & Therapy·Perry S Mongroo, Anil K Rustgi
Nov 22, 2011·Nature Medicine·Bogdan MateescuFatima Mechta-Grigoriou
Feb 23, 2013·Expert Review of Anti-infective Therapy·Richard D Sloan, Mark A Wainberg
Mar 19, 2013·Journal of Molecular Biology·Allan M Gurtan, Phillip A Sharp
May 21, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hassan FazilatyBabak Behnam
Nov 23, 2013·Cancer Letters·Xiangling FengYaguang Xi
Mar 22, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xuanyu ChenXiaoping Zhang
Apr 18, 2015·BMC Cancer·Silvia AntolínManuel Valladares-Ayerbes
Jul 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Nairi AbediMahdi Paryan
Feb 2, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yanping GaoLongbang Chen
Apr 10, 2016·Reviews in Medical Virology·Diogo Piedade, José Miguel Azevedo-Pereira
Apr 15, 2016·Journal of the National Cancer Institute·Bisrat G DebebWendy A Woodward
May 18, 2016·World Journal of Gastrointestinal Oncology·Azadeh AzizianJochen Gaedcke
Jun 5, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Kubra KabanJulide Akbuga
Jul 9, 2016·Nature Reviews. Neurology·Heather Wood
Sep 10, 2016·Human Genomics·Pei-Chun FanPao-Hsien Chu
Sep 20, 2016·Biochemical and Biophysical Research Communications·Wenxian CuiZhirong Kang
Oct 21, 2016·Nucleic Acid Therapeutics·Kubra KabanJulide Akbuga
Nov 7, 2016·Drug Discovery Today·Aditya GanjuMeena Jaggi
Nov 15, 2016·European Journal of Pharmacology·Zhexuan LinWenhong Luo
Jan 12, 2017·Genes·Weidan JiChangqing Su
Feb 10, 2017·Journal of Cellular Physiology·Prosenjit PaulSupriyo Chakraborty
Feb 18, 2017·Nature Reviews. Drug Discovery·Rajesha Rupaimoole, Frank J Slack
May 10, 2017·Gene Therapy·S T F Bots, R C Hoeben
Jun 18, 2017·Molecular Diagnosis & Therapy·Nina PetrovicEsma R Isenovic

❮ Previous
Next ❯

Citations

Feb 19, 2021·Pathology, Research and Practice·Zhi Xiong ChongWan Yong Ho
Mar 7, 2021·International Journal of Molecular Sciences·Rebecca RaueBernhard Brüne

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis