Modulation of the effects of glucocorticoids on collagen synthesis in fetal rat calvariae by insulin-like growth factor binding protein-2

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
B E KreamL G Raisz

Abstract

To test the hypothesis that insulin-like growth factors (IGFs) play a role in the response of bone to glucocorticoids, we determined the effects of cortisol on the incorporation of [3H]proline into collagenase-digestible protein (CDP) and noncollagen protein (NCP), the percent collagen synthesis, and the incorporation of [3H]thymidine into DNA of 21-day fetal rat calvariae cultured in the presence and absence of recombinant human insulin-like growth factor binding protein-2 (IGFBP-2). At 24 h, cortisol (100 nM) increased CDP labeling and the percent collagen synthesis, and these effects were blocked by IGFBP-2 (1000 nM). At 24 h, cortisol decreased the incorporation of [3H]thymidine into bone, which was not affected by the addition of IGFBP-2. At 48 h, cortisol (1000 nM) decreased CDP labeling, which was maintained in the presence of IGFBP-2. At 48 h, IGFBP-2 alone decreased basal levels of CDP and NCP labeling and the percent collagen synthesis. Our data suggest that endogenous IGFs maintain basal levels of collagen synthesis and mediate the early stimulatory effect of glucocorticoids on collagen synthesis in fetal rat calvariae. However, blocking endogenous IGFs does not abrogate the inhibitory effect of glucocorticoids on DN...Continue Reading

References

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Citations

May 13, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G OrtoftH Oxlund
Oct 12, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H W Woitge, B E Kream
Nov 9, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Dan Jia, Johan N M Heersche
Jan 2, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·T L McCarthyM Centrella
Jul 13, 2002·Journal of Biomedical Materials Research·François CornetJoseph Jeanfils
Jan 24, 1998·Journal of Cellular Biochemistry·M CentrellaT L McCarthy
Jun 5, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shreyasee AminSundeep Khosla
Mar 14, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shreyasee AminSundeep Khosla
Sep 24, 2010·Journal of Periodontal Research·Y TakenouchiK Ito
Apr 16, 2004·Endocrinology·Naibedya ChattopadhyayEdward M Brown
Mar 27, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·K McQueeney, C N Dealy
Nov 20, 2003·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Cheryl A Conover, Sundeep Khosla

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