PMID: 6193208Sep 1, 1983Paper

Modulation of the epidermal growth factor receptor of human keratinocytes by calcium ion

The Journal of Investigative Dermatology
E O'Keefe, R E Payne


Human keratinocytes grown in medium containing reduced calcium concentrations (0.07 mM) have been found to show altered morphology and decreased differentiation in comparison with cells grown in medium with physiologic calcium concentrations (1-2 mM). Since such alterations could be mediated by growth factors, we measured binding of [125I]epidermal growth factor (EGF). Neonatal keratinocytes were subcultured without feeder layers and grown to confluence in 1.1 mM calcium. Medium was changed and cells incubated for various periods in reduced calcium prior to binding assays. Scatchard plots of binding data showed a 5-fold increase in receptor number with no change in affinity (3 X 10(-9) M) after 4-24 h at 37 degrees C. Maximal binding occurred at 0.02-0.04 mM calcium and decreased sharply with increasing calcium concentrations. The increase could be prevented by calcium added soon after binding began to increase but was altered less after substantial elevation had occurred, although morphologic changes at reduced calcium concentrations were reversed within several hours. Substantial increases in binding of [125I]somatomedin C and [125I]concanavalin A were detected, but binding of [125I]pindalol, a beta-receptor ligand, was chang...Continue Reading


Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A McKannaS Cohen
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R W HolleyY C Yeh
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H HaiglerS Cohen
Jun 1, 1982·The Journal of Investigative Dermatology·E O'KeefeR Payne
Aug 1, 1980·The Journal of Investigative Dermatology·P Hawley-NelsonS H Yuspa
Feb 1, 1982·Journal of Cellular Physiology·M C TsaoR G Ham
Feb 1, 1981·The Journal of Investigative Dermatology·S H YuspaH Hennings

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Sep 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W F GreenleeR Osborne
Jan 1, 1991·Archives of Dermatological Research·M F te PasM Ponec
Feb 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·P G SacksD F Parsons
Nov 1, 1991·The American Journal of Physiology·F H KruszewskiR W Tucker
Oct 1, 1986·The Journal of Investigative Dermatology·P Hawley-NelsonS H Yuspa
Aug 1, 1985·The Journal of Investigative Dermatology·E J O'KeefeD T Woodley
May 1, 1990·The Journal of Investigative Dermatology·L B Nanney
Jun 1, 1992·The Journal of Investigative Dermatology·M PonecB J Vermeer
Feb 15, 1989·Archives of Biochemistry and Biophysics·M L Chiu, E J O'Keefe

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