PMID: 7547244Oct 1, 1995Paper

Modulation of the equilibrative nucleoside transporter by inhibitors of DNA synthesis

British Journal of Cancer
J PressaccoD W Hedley

Abstract

Expression of the equilibrative, S-(p-nitrobenzyl)-6-thioinosine (NBMPR)-sensitive nucleoside transporter (es), a component of the nucleoside salvage pathway, was measured during unperturbed growth and following exposure to various antimetabolites at growth-inhibitory concentrations. The probe 5-(SAENTA-x8)-fluorescein is a highly modified form of adenosine incorporating a fluorescein molecule. It binds. with high affinity and specificity to the (es) nucleoside transporter at a 1:1 stoichiometry, allowing reliable estimates of es expression by flow cytometry. Using a dual labelling technique which combined the vital DNA dye Hoechst-33342 and 5-(SAENTA-x8)-fluorescein, we found that surface expression of es approximately doubled between G1 and G2 + M phases of the cell cycle. To address the question of whether es expression could be modulated in cells exposed to drugs which inhibit de novo synthesis of nucleotides, cells were exposed to antimetabolite drugs having different modes of action. Hydroxyurea and 5-fluorouracil (5-FU), which inhibit the de novo synthesis of DNA precursors, produced increases in the expression of es. In contrast, cytosine arabinoside (ara-C) and aphidicolin, which directly inhibit DNA synthesis, produce...Continue Reading

Citations

Jan 26, 2005·Biochemical Society Transactions·I AymerichM Pastor-Anglada
Feb 24, 2001·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·R GuieuB Dussol
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·Vijaya L DamarajuCarol E Cass
Sep 6, 2007·Leukemia & Lymphoma·Sheryl A FlanaganKelly A Meckling
Aug 1, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J D YoungS A Baldwin
May 7, 2002·Journal of Virology·Richard A KatzAnna Marie Skalka
Aug 29, 2007·Journal of Physiology and Biochemistry·M Pastor-AngladaF J Casado
Nov 21, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Miguel A CabritaCarol E Cass
Jun 29, 2001·Japanese Journal of Cancer Research : Gann·T TakechiM Fukushima
Nov 6, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Laura ChowJohn R Mackey
Jan 15, 2005·Virus Research·Marçal Pastor-AngladaF Javier Casado
Dec 13, 2000·Gastroenterology·R ValdésM Pastor-Anglada

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