Modulation of the Hydration Water Around Monoclonal Antibodies on Addition of Excipients Detected by Terahertz Time-Domain Spectroscopy

Journal of Pharmaceutical Sciences
Vincent P WallaceJ Axel Zeitler

Abstract

Terahertz time-domain spectroscopy (THz-TDS) has been shown to detect overlapping extended hydration layers around proteins. Here, we used THz-TDS to detect modulation of the extended hydration layer around monoclonal antibodies (mAbs) by the introduction of commonly used excipients. Proline and sucrose altered the hydration layer around a mAb (mAb1), which was observed as a negative shift in the plateau in absorbance above ~100 mg/mL mAb1 (~70,000 water molecules per mAb); arginine had no effect. At lower concentrations of ~10 mg/mL mAb1 (~700,000 water molecules per mAb) proline and arginine modulated the hydration layer, which was observed as a negative shift in the relative absorbance, whereas sucrose had no effect. The changes in the extended hydration layer were not translated to shifts in the thermal stability or protein:protein interaction parameter. The hydration layer of a second mAb (mAb2) was further shown to be modulated by more complex formulations composed of two or more excipients; although the differences in terahertz absorbance were not predictive of viscosity or long-term stability. THz-TDS promises to be a useful tool for understanding a protein's interaction with excipients in solution and the challenge wil...Continue Reading

References

Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·S N Timasheff
Mar 3, 2004·Faraday Discussions·Vincent P WallaceDonald D Arnone
Mar 1, 2005·Journal of Pharmaceutical Sciences·Clare J StrachanThomas Rades
Apr 28, 2006·Protein Science : a Publication of the Protein Society·Jing XuS James Allen
Dec 5, 2006·International Journal of Pharmaceutics·Fei TianJ Howard Rytting
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Simon EbbinghausMartina Havenith
Jun 12, 2008·Optics Express·Withawat WithayachumnankulDerek Abbott
Jul 24, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Louise HoJ Axel Zeitler
Nov 28, 2008·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Vincent P WallaceCaroline Reid
Aug 6, 2009·Optics Express·Philip C AshworthVincent P Wallace
Nov 26, 2009·Biologicals : Journal of the International Association of Biological Standardization·Reinhard BolliPeter Lerch
Aug 26, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Youssef El KhouryPetra Hellwig
Dec 31, 2010·Biophysical Journal·Yiwen SunEmma Pickwell-Macpherson
Jul 27, 2011·Journal of Pharmaceutical Sciences·Feng HeVladimir I Razinkov
Jan 1, 2011·Faraday Discussions·Gudrun NiehuesMartina Havenith
Jun 28, 2012·Journal of Pharmaceutical Sciences·Dean C Ripple, Mariana N Dimitrova
Sep 17, 2013·Protein Science : a Publication of the Protein Society·Rajeev Yadav, Pratik Sen
Dec 18, 2013·The Journal of Physical Chemistry. a·Jordan W ByeRobert J Falconer
Dec 18, 2013·Journal of the American Chemical Society·John T KingKevin J Kubarych
Jan 17, 2014·Nature Communications·Gheorghe AcbasA G Markelz
Mar 4, 2014·Molecular Pharmaceutics·Juraj SibikJ Axel Zeitler
Apr 2, 2014·The Journal of Physical Chemistry. B·Jordan W Bye, Robert J Falconer
Apr 3, 2014·Molecular Pharmaceutics·Naoto InoueKentaro Shiraki
Jul 24, 2014·IEEE Journal of Biomedical and Health Informatics·Caroline B ReidVincent P Wallace
Dec 17, 2014·Applied Spectroscopy·Edward P J Parrott, J Axel Zeitler
Apr 4, 2013·The Journal of Physical Chemistry Letters·Lucia ComezDaniele Fioretto

❮ Previous
Next ❯

Citations

Aug 5, 2016·The Journal of Physical Chemistry. B·Guillaume SerinChristophe Coudret
Oct 5, 2021·The Journal of Physical Chemistry. B·D K GeorgeA G Markelz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.