Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics

The British Journal of Nutrition
Vivian Cristina da Cruz RodriguesAdriane Elisabete Costa Antunes

Abstract

The aim of the present work was to compare the capacity to modulate the intestinal microbiota and the production of metabolites after 14 d administration of a commercial dietary supplement and a manufactured ice cream, both containing the same quantity of inulin and the same viable counts of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis BB-12, using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model. Samples of the colonic contents were evaluated microbiologically by real-time quantitative PCR (qRT-PCR) and next-generation sequencing and chemically by the production of SCFA (acetate, propionate and butyrate) and ammonium ions ($\text{NH}_4^ + $). Statistical analyses were carried out for all the variables using the two-way ANOVA followed by the Tukey multiple comparisons test (P < 0·05) for metabolite production, qRT-PCR and the bioinformatics analysis for microbiota diversity. Dietary supplement and ice cream were able to deliver the probiotic L. acidophilus and B. animalis to the simulated colon and modulate the microbiota, increasing beneficial micro-organisms such as Bifidobacterium spp., Bacteroides spp. and Faecalibacterium spp. for dietary supplement administration, and Lactobacillus s...Continue Reading

References

Jun 1, 1995·The Journal of Nutrition·G R Gibson, M B Roberfroid
Nov 9, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M F BöttcherB Björkstén
Sep 17, 2002·Biometrics·Anne Chao, John Bunge
Feb 14, 2003·Lancet·Francisco Guarner, Juan-R Malagelada
Feb 25, 2003·Journal of Medical Entomology·Marcelo B LabrunaJames E Keirans
May 13, 2003·The Proceedings of the Nutrition Society·Sandra Macfarlane, George T Macfarlane
Dec 7, 2005·FEMS Microbiology Ecology·Tom Van de WieleWilly Verstraete
Dec 8, 2005·Applied and Environmental Microbiology·Catherine Lozupone, Rob Knight
Jan 24, 2007·Journal of Applied Microbiology·T van de WieleW Verstraete
Apr 13, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·W von EngelhardtM Lechner-Doll
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Oct 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Harry SokolPhilippe Langella
Jul 16, 2009·Environmental Microbiology·Julien TapMarion Leclerc
Nov 17, 2009·Bioinformatics·J Gregory CaporasoRob Knight
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
May 11, 2010·International Journal of Food Microbiology·S PossemiersT Van de Wiele
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·J Gregory CaporasoRob Knight
Aug 7, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mireille AndriamihajaFrançois Blachier
Aug 17, 2010·Bioinformatics·Robert C Edgar
Aug 18, 2010·International Journal of Cancer. Journal International Du Cancer·Jean-Marc BlouinSylvie Bortoli
Feb 1, 2011·International Journal of Food Microbiology·Dominika ŚwiąteckaKostyra Henryk
Feb 18, 2011·Gut Microbes·Sarah O'FlahertyJames Versalovic
May 28, 2011·Genes & Nutrition·Jacoline GerritsenWillem M de Vos
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Oct 22, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Henrike M HamerKristin Verbeke
Nov 22, 2011·Nature Reviews. Microbiology·Peter A BronMichiel Kleerebezem
Nov 29, 2011·Molecular Nutrition & Food Research·Karen WindeyKristin Verbeke
Jan 1, 2010·Annual Review of Food Science and Technology·Mary Ellen Sanders, Maria L Marco
Apr 4, 2012·Journal of AOAC International·George T Macfarlane, Sandra Macfarlane
Oct 24, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jane M M Natividad, Elena F Verdu
Nov 14, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Karen P ScottSylvia H Duncan
Nov 28, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Anne-Marie DavilaDaniel Tomé
Dec 12, 2012·Journal of Gastroenterology and Hepatology·Takehide FujimotoAkira Andoh
Apr 20, 2013·Current Opinion in Microbiology·Arya Khosravi, Sarkis K Mazmanian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.