Modulation of the NO pathway during short or prolonged blood reperfusion following ischaemia in a heterotopic rat heart transplantation model

Transplantation Proceedings
Martine DesroisM Bernard

Abstract

Ischemia-reperfusion injury plays a major role in graft dysfunction following transplantation. Extensive research has demonstrated that nitric oxide (NO) plays a fundamental role to protect the heart against this injury. Consequently, we quantified NO synthase (NOS) isoform protein levels in a rat heart transplant model during short and prolonged reperfusion following ischemia. Experiments were performed using a modified Lewis to Lewis heterotopic abdominal heart transplantation with a total ischemic time of 3 hours followed by 1 or 24 hours of blood reperfusion (n = 12). Heart function, as represented by the rate pressure product, increased from 7912 +/- 489 to 27067 +/- 9982 mm Hg/min (mean +/- SEM, short vs prolonged reperfusion, P = .0027). NOS isoform protein levels determined using Western blotting of freeze-clamped hearts were compared to baseline values. eNOS protein levels were significantly lower during short reperfusion compared to the basal value (P = .0077) or to prolonged reperfusion (P = .004), returning to the basal value after 24 hours of reflow. iNOS protein was not detected in the basal condition or after 1 hour of reflow, but was present after 24 hours of reflow (P = .0001 vs basal value and 1-hour reflow). ...Continue Reading

References

Aug 1, 1994·The Journal of Clinical Investigation·X YangP J Cannon
Feb 19, 1998·Circulation Research·A GödeckeJ Schrader
Oct 13, 1998·Journal of the American College of Cardiology·H DrexlerG Hasenfuss
Jul 8, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·M BernardP J Cozzone
May 10, 2001·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·T CausM Bernard
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Song-Jung KimStephen F Vatner

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Citations

Sep 22, 2012·Scandinavian Cardiovascular Journal. Supplement·Ari A MennanderMatti Tarkka
Nov 28, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Martine DesroisMonique Bernard
Dec 18, 2002·Journal of Cardiothoracic and Vascular Anesthesia·Jin Hee KimChung Il Noh
Nov 21, 2007·Transplantation Proceedings·J NozyńskiM Zembala
Sep 11, 2010·The Journal of Surgical Research·Claudio MuscariCarlo Guarnieri

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