Modulation of the secretion of mesenchymal stem cell immunoregulatory factors by hydrolyzed fish collagen

Experimental and Therapeutic Medicine
Chao Liu, Jiao Sun

Abstract

The aim of the present study was to investigate the possible immunomodulatory effects of osteogenically differentiated bone marrow mesenchymal stem cells induced by hydrolyzed fish collagen. Marine biomaterials have attracted significant attention for their environmental friendliness and renewability. Hydrolyzed fish collagen (HFC) has been discovered to induce the osteoblastic differentiation of stem cells, which underlies the foundation for its application in tissue engineering. Stem cells and their biomaterial carriers face acute immune rejection mediated by host macrophages. A potential strategy for combatting rejection in stem cell therapy is to modify the polarization of macrophages. However, whether HFC-induced mesenchymal stem cells maintain their immunomodulatory ability remains to be determined. To understand this phenomenon, a co-culture model of direct contact was established between bone marrow mesenchymal stem cells (BMSCs) and RAW264.7 macrophages, where the secretion of nitrous oxide from macrophages was measured using Griess colorimetric assay. ELISAs were performed to measure the secretion of interleukin (IL)-1β, IL-6, transforming growth factor (TGF)-β and IL-10, whilst reverse transcription-quantitative PCR ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 23, 2007·Cardiovascular Research·Jiang ShujiaMuhammad Ashraf
Mar 24, 2009·Annual Review of Immunology·Charles A Dinarello
Sep 24, 2009·Experimental Hematology·Jaehyup Kim, Peiman Hematti
Feb 26, 2010·Clinical Pharmacology and Therapeutics·N Nishimoto
May 25, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Charles S Wallace, George A Truskey
Aug 25, 2011·Stem Cell Research & Therapy·Michelle M DuffyMatthew D Griffin
Mar 2, 2012·The Journal of Clinical Investigation·Antonio Sica, Alberto Mantovani
Feb 23, 2013·Critical Reviews in Immunology·Charles D Mills
Jun 19, 2014·Journal of Cardiovascular Pharmacology and Therapeutics·Tamar Ben-MordechaiJonathan Leor
Oct 24, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Amirbahman RahimzadehAbolfazl Akbarzadeh
Jan 20, 2015·Current Opinion in Immunology·Sharad K Mittal, Paul A Roche
Jun 28, 2015·Immunology Letters·Maria Ester Bernardo, Willem E Fibbe
Jul 27, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Wei DengLingyun Sun
Nov 13, 2015·European Journal of Immunology·Annunziata CrupiLaura Teodori
Nov 22, 2016·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A PolymeriD Kaigler
Jan 11, 2018·Journal of Cellular Physiology·Abbas Shapouri-MoghaddamAmirhossein Sahebkar
Jun 15, 2018·The Journal of Biological Chemistry·Lilli ArndtRalph Burkhardt
Aug 14, 2018·Current Opinion in Pharmacology·Chloe L Rackham, Peter M Jones
Sep 27, 2018·Histochemistry and Cell Biology·Benjamin Mattes, Steffen Scholpp
Feb 28, 2019·Journal of Applied Biomaterials & Functional Materials·Patrícia VarelaGianluca Ciardelli
Apr 18, 2019·Immunity·Eduard Batlle, Joan Massagué
Jun 30, 2019·International Journal of Molecular Sciences·Raquel Costa-AlmeidaManuela E Gomes
Oct 17, 2019·Materials·Somasundar ManthaSimon D Tran
Mar 3, 2020·Advances in Wound Care·Emily H SteenSundeep G Keswani

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
PCR
ELISAs

Software Mentioned

SPSS
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.