Modulation of the Senescence-Associated Inflammatory Phenotype in Human Fibroblasts by Olive Phenols

International Journal of Molecular Sciences
Beatrice MenicacciLisa Giovannelli

Abstract

Senescent cells display an increase in the secretion of growth factors, inflammatory cytokines and proteolytic enzymes, termed the "senescence-associated-secretory-phenotype" (SASP), playing a major role in many age-related diseases. The phenolic compounds present in extra-virgin olive oil are inhibitors of oxidative damage and have been reported to play a protective role in inflammation-related diseases. Particularly, hydroxytyrosol and oleuropein are the most abundant and more extensively studied. Pre-senescent human lung (MRC5) and neonatal human dermal (NHDF) fibroblasts were used as cellular model to evaluate the effect of chronic (4-6 weeks) treatment with 1 μM hydroxytyrosol (HT) or 10 μM oleuropein aglycone (OLE) on senescence/inflammation markers. Both phenols were effective in reducing β-galactosidase-positive cell number and p16 protein expression. In addition, senescence/inflammation markers such as IL-6 and metalloprotease secretion, and Ciclooxigenase type 2 (COX-2) and α-smooth-actin levels were reduced by phenol treatments. In NHDF, COX-2 expression, Nuclear Factor κ-light-chain-enhancer of activated B cells (NFκB) protein level and nuclear localization were augmented with culture senescence and decreased by OLE...Continue Reading

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Citations

Aug 8, 2018·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Beatrice MenicacciAlessandra Mocali
Jun 28, 2019·British Journal of Pharmacology·Lukas SchwingshacklGeorg Hoffmann
Apr 17, 2020·Nutrients·Marica CarielloAntonio Moschetta
Apr 21, 2020·Frontiers in Cell and Developmental Biology·Amanpreet KaurCordula M Stover
Mar 23, 2019·International Journal of Molecular Sciences·Tiziana LarussaFrancesco Luzza
Apr 7, 2020·Mechanisms of Ageing and Development·Daria KhaltourinaAnca Ioviţă
Mar 7, 2021·Antioxidants·Jing LuoDongmin Liu
Jan 12, 2022·The FEBS Journal·Lei ZhangPaul D Robbins

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Methods Mentioned

BETA
nuclear translocation
protein assay
zymography
electrophoresis
ELISA

Software Mentioned

ImageJ
image J

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