Modulation of tumor cell migration, invasion and cell-matrix adhesion by human monopolar spindle-one-binder 2

Oncology Reports
Wenjuan WuWeigan Shen

Abstract

Human monopolar spindle-one-binder 2 (hMOB2) is a member of the hMOB family of proteins, and it has been reported to regulate the nuclear-Dbf2-related kinase (NDR) activation. However, the function of hMOB2 expression in tumor cell adhesion and motility has not been addressed. Herein, the lentiviral-mediated overexpression and the knockdown of hMOB2 in HepG2 and SMMC-7721 cells was established. It was demonstrated that overexpression of hMOB2 significantly reduced the cell motility and enhanced the cell-matrix adhesion, while the hMOB2 knockdown decreased not only the cell motility, but also the cell-matrix adhesion. Immunofluorescence results showed that both hMOB2 overexpression and knockdown altered assembly of the focal adhesions and the actin cytoskeleton rearrangement. Furthermore, the focal adhesion kinase (FAK)-Src-paxillin signal pathway activated by hMOB2 was confirmed to be involved in controlling the cell motility and the cell-matrix adhesion. These results demonstrated that the altered cell-matrix adhesion and cell motility induced by hMOB2 expression was caused by the assembly of focal adhesions as well as the actin cytoskeleton rearrangement through the activation of the FAK-Src-paxillin signal pathway, unveiling...Continue Reading

References

Feb 7, 1998·Science·A Hall
Dec 15, 2000·Oncogene·J D BjorgeD J Fujita
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·B GeigerK M Yamada
Jan 27, 2004·Nature Cell Biology·Donna J WebbAlan F Horwitz
Aug 17, 2004·The Journal of Biological Chemistry·Oliver StorkHans-Christian Pape
May 3, 2006·The Journal of Cell Biology·Pirta Hotulainen, Pekka Lappalainen
Aug 22, 2006·Current Opinion in Cell Biology·Satyajit K Mitra, David D Schlaepfer
Aug 29, 2006·Journal of Molecular Biology·Serge MrkobradaFrank Sicheri
Sep 26, 2006·Biochemical Pharmacology·Maroesja J van Nimwegen, Bob van de Water
Nov 21, 2007·Seminars in Cancer Biology·John G LockStaffan Strömblad
Jan 27, 2009·Cancer Metastasis Reviews·Jihe Zhao, Jun-Lin Guan
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Benjamin GeigerAlexander D Bershadsky
Apr 14, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Daniella Pereira Crosara-AlbertoCláudia Raquel Cantarelli Costa
May 9, 2009·International Journal of Oncology·Severine Van SlambrouckWim F A Steelant
Aug 21, 2009·Nature·Matthias MachacekGaudenz Danuser
Oct 17, 2009·Cell and Tissue Research·Ling-Yu LiuSeng-Sheen Fan
Dec 29, 2009·Experimental Cell Research·Tohru Ishikawa, Randall H Kramer
May 18, 2010·Nature Cell Biology·Stephanie I FraleyDenis Wirtz
Jan 18, 2011·FEBS Letters·Cheng-Han LinSeng-Sheen Fan
May 5, 2011·Cellular Signalling·Alexander Hergovich
Sep 1, 2011·Journal of Cell Science·Nathan J LanningChristin Carter-Su
Feb 15, 2012·Journal of Cell Science·Alexandra TavaresHelena Soares
Mar 29, 2012·Journal of Cell Science·Claudia FlorindoAlvaro A Tavares
Dec 12, 2012·Biochemical and Biophysical Research Communications·Jin LiuHaifeng Duan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.