Modulation of violacein production and phenotypes associated with biofilm by exogenous quorum sensing N-acylhomoserine lactones in the marine bacterium Pseudoalteromonas ulvae TC14

Microbiology
Armande Mireille AyéMaëlle Molmeret

Abstract

Various phenotypes ranging from biofilm formation to pigment production have been shown to be regulated by quorum sensing (QS) in many bacteria. However, studies of the regulation of pigments produced by marine bacteria in saline conditions and of biofilm-associated phenotypes are scarcer. This study focuses on the demonstration of the existence of a QS communication system involving N-acylhomoserine lactones (AHLs) in the Mediterranean Sea strain Pseudoalteromonas ulvae TC14. We have investigated whether TC14 produces the violacein pigment, and whether intrinsic or exogenous AHLs could influence its production and modulate biofilm-associated phenotypes. Here, we demonstrate that the purple pigment produced by TC14 is violacein. The study shows that in planktonic conditions, TC14 produces more pigment in the medium in which it grows less. Using different approaches, the results also show that TC14 does not produce intrinsic AHLs in our conditions. When exogenous AHLs are added in planktonic conditions, the production of violacein is upregulated by C6-, C12-, 3-oxo-C8 and 3-oxo-C12-HSLs (homoserine lactones), and downregulated by 3-oxo-C6-HSL. In sessile conditions, 3-oxo-C8-HSL upregulates the production of violacein. The study...Continue Reading

References

Jul 1, 1992·Antimicrobial Agents and Chemotherapy·H AnwarJ W Costerton
May 1, 1991·Antimicrobial Agents and Chemotherapy·B GiwercmanJ W Costerton
Apr 28, 1981·Biochemistry·A EberhardN J Oppenheimer
Aug 1, 1996·Applied and Environmental Microbiology·S G JamesS Kjelleberg
Nov 1, 1996·Journal of Bacteriology·M GivskovS Kjelleberg
Oct 23, 1997·International Journal of Systematic Bacteriology·J C MakemsonJ F Case
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·E C PesciB H Iglewski
Aug 19, 2000·Infection and Immunity·T R de Kievit, B H Iglewski
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Feb 7, 2001·Applied and Environmental Microbiology·J B AndersenM Givskov
Feb 13, 2001·Microbiology·I Sutherland
Aug 9, 2001·International Journal of Systematic and Evolutionary Microbiology·S EganS Kjelleberg
Aug 29, 2001·FEMS Microbiology Reviews·N A WhiteheadG P Salmond
Sep 7, 2001·Annual Review of Microbiology·M B Miller, B L Bassler
Jun 12, 2002·Current Opinion in Microbiology·Staffan Kjelleberg, Soeren Molin
Jun 28, 2002·Cell·Bonnie L Bassler
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·Clay Fuqua, E Peter Greenberg
Feb 4, 2003·Journal of Bacteriology·Jenée N Smith, Brian M M Ahmer
Mar 20, 2003·Journal of Bacteriology·Victoria E WagnerBarbara H Iglewski
Jan 20, 2004·Journal of Bacteriology·Maurizio LabbateStaffan Kjelleberg
Mar 10, 2004·Applied and Environmental Microbiology·Carsten MatzKlaus Jürgens
Jul 29, 2004·Journal of Microbiological Methods·Robert J C McLeanClay Fuqua
Sep 29, 2004·FEMS Microbiology Reviews·Ruth DanielsJan Michiels
Sep 15, 2005·Journal of the American Chemical Society·Grant D GeskeHelen E Blackwell
Nov 18, 2006·Applied Microbiology and Biotechnology·Thunyarat Pongtharangku, Ali Demirci
Jan 20, 2007·FEMS Microbiology Letters·Laura Steindler, Vittorio Venturi
May 22, 2007·BMC Microbiology·Jintae LeeThomas K Wood
Aug 19, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Srdjan StepanovićFilip Ruzicka
Oct 12, 2007·Biotechnology and Applied Biochemistry·Nelson DuránDorival Martins
Oct 31, 2007·Marine Biotechnology·Shuichi YadaKeiich Enomoto

❮ Previous
Next ❯

Citations

Mar 23, 2017·Frontiers in Microbiology·Giulia DevescoviVittorio Venturi
Nov 20, 2019·Microbial Ecology·Mahmoud HayekMaëlle Molmeret
Mar 19, 2021·Critical Reviews in Biotechnology·HyunA ParkKwon-Young Choi
Aug 17, 2021·World Journal of Microbiology & Biotechnology·Nelson DuránLjubica Tasic
Sep 30, 2021·Journal of Microbiology and Biotechnology·Aqsa AhmedZhonghu Bai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.