Modulations of DNA Contacts by Linker Histones and Post-translational Modifications Determine the Mobility and Modifiability of Nucleosomal H3 Tails

Molecular Cell
Alexandra StützerWolfgang Fischle

Abstract

Post-translational histone modifications and linker histone incorporation regulate chromatin structure and genome activity. How these systems interface on a molecular level is unclear. Using biochemistry and NMR spectroscopy, we deduced mechanistic insights into the modification behavior of N-terminal histone H3 tails in different nucleosomal contexts. We find that linker histones generally inhibit modifications of different H3 sites and reduce H3 tail dynamics in nucleosomes. These effects are caused by modulations of electrostatic interactions of H3 tails with linker DNA and largely depend on the C-terminal domains of linker histones. In agreement, linker histone occupancy and H3 tail modifications segregate on a genome-wide level. Charge-modulating modifications such as phosphorylation and acetylation weaken transient H3 tail-linker DNA interactions, increase H3 tail dynamics, and, concomitantly, enhance general modifiability. We propose that alterations of H3 tail-linker DNA interactions by linker histones and charge-modulating modifications execute basal control mechanisms of chromatin function.

References

Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BednarC L Woodcock
Dec 28, 1999·Molecular and Cellular Biology·J E HerreraM Bustin
Nov 4, 2000·The Journal of Biological Chemistry·A GunjanD T Brown
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·D AngelovJ J Hayes
Feb 13, 2002·Molecular and Cellular Biology·Brian D StrahlC David Allis
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Jeffrey C Hansen
Jun 17, 2003·Molecular and Cellular Biology·Yuhong FanArthur I Skoultchi
Feb 27, 2004·The Journal of Biological Chemistry·Michael J HendzelJohn P H Th'ng
Aug 13, 2004·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·A A Travers
Mar 8, 2005·Molecular Cell·Michael BustinJae-Hwan Lim
Mar 1, 2006·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Christopher L WoodcockYuhong Fan
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gaurav Arya, Tamar Schlick
Aug 19, 2007·Oncogene·S MujtabaM-M Zhou
Sep 2, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Anne K Voss, Tim Thomas
May 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sajad Hussain SyedStefan Dimitrov
May 28, 2010·Epigenetics : Official Journal of the DNA Methylation Society·Jihong ChenQiao Li
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Menno P CreyghtonRudolf Jaenisch
Feb 16, 2011·Cell Research·Andrew J Bannister, Tony Kouzarides
Oct 25, 2011·Nucleic Acids Research·He FangJeffrey J Hayes
Mar 8, 2012·Experimental Cell Research·Sergei A Grigoryev, Christopher L Woodcock
Jun 23, 2012·Nature Reviews. Molecular Cell Biology·Karolin LugerDavid J Tremethick
Jul 17, 2012·Nature Structural & Molecular Biology·Stamatios LiokatisPhilipp Selenko
Aug 13, 2013·Nature Structural & Molecular Biology·Manuela DelvecchioDaniel Panne
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bing-Rui ZhouYawen Bai
Jan 15, 2014·Biochimica Et Biophysica Acta·Bryan M Turner
Apr 17, 2014·Chromosoma·Kazuhiro MaeshimaTadasu Nozaki
May 17, 2014·Genes & Development·Marie-Eve LalondeJacques Côté

❮ Previous
Next ❯

Citations

Jun 4, 2016·Molecular Cell·Alexey A SoshnevC David Allis
Jun 14, 2016·Current Opinion in Structural Biology·Heyi Zhang, Hugo van Ingen
Nov 5, 2016·Protein Science : a Publication of the Protein Society·Ciara KynePeter B Crowley
Aug 17, 2016·Scientific Reports·Zhenhai Li, Hidetoshi Kono
Jul 15, 2017·Nature Communications·Emma A MorrisonCatherine A Musselman
Feb 22, 2018·Angewandte Chemie·ShengQi XiangHugo van Ingen
Nov 16, 2017·Nature Communications·Jovylyn GatchalianTatiana G Kutateladze
Aug 24, 2018·Systems Biology in Reproductive Medicine·Jovita Mezquita-Pla
Jan 17, 2019·Journal of Biochemistry·Kyoko Hiragami-Hamada, Jun-Ichi Nakayama
May 14, 2016·G3 : Genes - Genomes - Genetics·Michael SeymourZachary A Lewis
Mar 19, 2019·Annual Review of Biophysics·Beat Fierz, Michael G Poirier
Oct 12, 2017·Nature Reviews. Molecular Cell Biology·Dmitry V FyodorovYawen Bai
Jan 21, 2020·Nucleic Acids Research·Kathrin LehmannJörg Langowski
Jan 20, 2019·International Journal of Molecular Sciences·Amit KumarJochen Balbach
Oct 12, 2018·Experimental & Molecular Medicine·Jihye AhnJeong Hyeon Park
Aug 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ayako FurukawaYoshifumi Nishimura
Oct 18, 2018·Nature Communications·Stephanie LinkSandra B Hake
Oct 17, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tyler M WeaverCatherine A Musselman
Mar 17, 2019·International Journal of Molecular Sciences·Philipp Selenko
Jan 1, 2019·Nucleic Acids Research·Katelyn E ConnellyEmily C Dykhuizen
Oct 4, 2017·Nucleic Acids Research·Kevin J MurphyJeffrey J Hayes
Feb 25, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Erina KakumuKaoru Sugasawa
Nov 7, 2019·Scientific Reports·Deb Ranjan BanerjeeJonathan T Sczepanski
Nov 27, 2019·Nature Communications·Christian AlbigCatherine Regnard
Oct 18, 2020·Nature Communications·Alexandra StützerHenning Urlaub
Dec 15, 2020·Journal of Molecular Biology·Jan Huertas, Vlad Cojocaru
Dec 7, 2020·Current Opinion in Structural Biology·Yunhui PengAnna R Panchenko
Dec 25, 2019·International Journal of Biological Macromolecules·Biling HuangYufen Zhao
Feb 2, 2021·Angewandte Chemie·Sevastyan O RabdanoChristopher P Jaroniec
Feb 9, 2021·Trends in Biochemical Sciences·Mohamed GhoneimCatherine A Musselman
Jan 13, 2021·Nature Communications·Benjamin J E MartinLeAnn J Howe
Feb 20, 2021·IScience·Catherine A Musselman, Tatiana G Kutateladze
Apr 16, 2021·Nucleic Acids Research·Emma A MorrisonCatherine A Musselman
Jun 20, 2021·Journal of Biomedical Science·Chia-Ling HsuCheng-Fu Kao
Jun 30, 2021·The Journal of Physical Chemistry Letters·Mohamad ZandianChristopher P Jaroniec

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Cold Spring Harbor Symposia on Quantitative Biology
E Morton BradburyC Crane-Robinson
Proceedings of the National Academy of Sciences of the United States of America
L FreemanA P Wolffe
Molecular and Cellular Biochemistry
D Doenecke, D Gallwitz
Proceedings of the National Academy of Sciences of the United States of America
D AngelovJ J Hayes
Biochimica Et Biophysica Acta
Cédric GrauffelAnnick Dejaegere
© 2022 Meta ULC. All rights reserved