PMID: 8586484Jul 1, 1995Paper

Modulations of histamine release from mast cells by interleukin-2 is affected by nedocromil sodium

International Journal of Immunopharmacology
E RubinchikF Levi-Schaffer

Abstract

We have previously demonstrated that histamine release from immunologically activated mast cells (MC) is enhanced by their preincubation (1 h) with interleukin-2(IL-2), and that IL-2 induces slow-chronic histamine release by MC in long-term cultures (6 days). In the present study we assessed whether nedocromil sodium can interfere with IL-2 modulation of MC histamine release. IL-2 enhancing effects nedocromil sodium activity were studied in cocultures of rat peritoneal MC with 3T3 fibroblasts (MC/3T3). MC/3T3 were preincubated for 1 h with IL-2 (50 micrograms/ml) and activated with either rabbit anti-rat IgE or compound 48/80. In chronic experiments MC/3T3 were long-term (5-6 days) incubated with IL-2 (50 micrograms/ml). Nedocromil sodium was used at 10(-5) M. MC activation both when added during the preincubation period (no tachyphylaxis was present) and when added together with the MC activators (30-50% inhibition). Washing out IL-2 before addition of the anti-IgE antibodies did not affect its histamine-release enhancing activity. Removal of nedocromil sodium before addition of the stimulus completely abrogated its effect. Continuous presence of IL-2 in the culture medium enhanced spontaneous histamine release by 37% and this...Continue Reading

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Citations

May 30, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E EberP D Sly
May 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F Levi-Schaffer, V B Weg
Sep 14, 2007·Cancer Investigation·Theodore F LoganMacy I Levine
Jul 27, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Elaine Zayas Marcelino da SilvaConstance Oliver
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C K OhS H Cho

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