Modulators of internal Ca2+ stores and the spontaneous electrical and contractile activity of the guinea-pig renal pelvis

British Journal of Pharmacology
R J LangH Suzuki

Abstract

1. The role of internal Ca(2+) stores in the generation of the rhythmic electrical and contractile activity in the guinea-pig proximal renal pelvis was examined using intracellular microelectrode and muscle tension recording techniques. 2. Ryanodine (30 microM) transiently increased contraction amplitude, while caffeine (0.5 - 3 mM) reduced contraction amplitude and frequency. Contractility was also reduced by 2-aminoethoxy-diphenylborate (2-APB 60 microM), xestospongin C (1 microM), U73122 (5 microM) and neomycin (4 mM), blockers of IP(3)-dependent release from Ca(2+) stores. 3. 60 mM K(+) saline-evoked contractions were reduced by caffeine (1 mM), U73122 (5 microM) and neomycin (4 mM), but little affected by ryanodine or 2-APB (60 microM). 4. Spontaneous action potentials consisting of an initial spike followed by a long plateau were recorded (frequency 8.6+/-1.0 min(-1)) in small urothelium-denuded strips of proximal renal pelvis. 5. Action potential discharge was blocked in 75 and 35% of cells by 2-APB (60 microM) and caffeine (1 mM), respectively. In the remaining cells, only a truncation of the plateau phase was observed. 6. Cyclopiazonic acid (CPA 10 microM for 10 - 180 min), blocker of CaATPase, transiently increased co...Continue Reading

References

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Citations

Jun 12, 2009·Clinical and Experimental Pharmacology & Physiology·Richard J LangHikaru Suzuki
Oct 6, 2005·Journal of Cellular and Molecular Medicine·R J Lang, M F Klemm
Jan 13, 2006·The Journal of Urology·Richard J LangBetty Exintaris
Jul 21, 2006·Biology of Reproduction·Marco MeweRalf Middendorff
Feb 27, 2010·Journal of Cellular and Molecular Medicine·Hikaru Hashitani, Richard J Lang
Sep 25, 2012·Neurourology and Urodynamics·Antonina Di BenedettoCarlo Magno
Dec 17, 2004·Pharmacological Reviews·Régent LaporteIsmail Laher

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