PMID: 9548418Apr 21, 1998Paper

Modulatory function on autologous myeloid progenitor cells of clonal T-lymphocytes following autologous bone marrow transplantation

European Journal of Haematology
P HoklandB Nielsen

Abstract

We have studied the regulatory capacity of clonal T-lymphocytes from patients undergoing autologous bone marrow transplantation (ABMT) on the generation of CFU-GM from their harvest marrow preparations. To this end, T-lymphocytes from peripheral blood from 5 patients undergoing ABMT isolated 10 d before and 7, 14 and 28 d post-ABMT were placed in limiting dilution conditions (384 wells for each patient at each time point) and polyclonally stimulated. From more than 1600 wells with growth from the 5 patients, preparations from more than 900 wells could be expanded (range between patients 33-452) and identified by immunophenotyping (IP) and flow cytometry (FCM) by their exclusive expression of CD4 or CD8. This was significantly fewer than seen in normal donors, especially so at d 7 and 14 post-ABMT The ratio between CD4+ and CD8+ clones varied between 0.6 and 2.8 (median 1.3) and was significantly lower in the patients compared to normal donors (median 3.1; range 3.0-6.5). When the clonal T-cell preparations were co-cultured with autologous bone marrow cells obtained at the time of harvest and depleted for T-lymphocytes, the vast majority of both CD4+ and CD8+ clones exerted a clear enhancement on the CFU-GM growth with no relati...Continue Reading

References

May 1, 1992·British Journal of Haematology·S V DaviesJ A Whittaker
Nov 1, 1994·European Journal of Haematology·B Nielsen, P Hokland
Jan 1, 1994·Annual Review of Immunology·R A Seder, W E Paul
May 8, 1997·The New England Journal of Medicine·N S Young, J Maciejewski

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