Modules for C-terminal epitope tagging of Tetrahymena genes.

Journal of Microbiological Methods
Kensuke KataokaKazufumi Mochizuki

Abstract

Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies.

References

Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yuhua ShangMartin A Gorovsky
Oct 3, 2002·The Journal of Cell Biology·Yali DouMartin A Gorovsky
Nov 4, 2004·Journal of Cell Science·Josef Loidl, Harry Scherthan
Apr 14, 2006·Molecular Biology of the Cell·Dorota WlogaJacek Gaertig
Jul 3, 2007·Nature Structural & Molecular Biology·Suzanne R Lee, Kathleen Collins
Dec 17, 2009·The Journal of Cell Biology·Chad G PearsonMark Winey

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Citations

Jan 15, 2015·The Journal of Eukaryotic Microbiology·Yasuhiro FukudaRonald E Pearlman
Jul 29, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kensuke KataokaKazufumi Mochizuki
Jun 9, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masaaki IwamotoTokuko Haraguchi
Nov 14, 2018·Critical Reviews in Biotechnology·María E ElgueroAlejandro D Nusblat
Jun 9, 2016·Genetics·Marisa D RuehleChad G Pearson
Jul 25, 2015·Genetics·Azusa Hayashi, Kazufumi Mochizuki
Nov 12, 2020·Journal of Cell Science·Miao TianJosef Loidl

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Methods Mentioned

BETA
tandem affinity protein purification
PCR

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