DOI: 10.1101/004556Apr 25, 2014Paper

MOHCA-seq: RNA 3D models from single multiplexed proximity-mapping experiments

BioRxiv : the Preprint Server for Biology
Clarence Yu ChengRhiju Das

Abstract

Large RNAs control myriad biological processes but challenge tertiary structure determination. We report that integrating multiplexed •OH cleavage analysis with tabletop deep sequencing (MOHCA-seq) gives nucleotide-resolution proximity maps of RNA structure from single straightforward experiments. After achieving 1-nm resolution models for RNAs of known structure, MOHCA-seq reveals previously unattainable 3D information for ligand-induced conformational changes in a double glycine riboswitch and the sixth community-wide RNA puzzle, an adenosylcobalamin riboswitch.

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