Molded continuous poly(styrene-co-divinylbenzene) rod as a separation medium for the very fast separation of polymers. Comparison of the chromatographic properties of the monolithic rod with columns packed with porous and non-porous beads in high-performance liquid chromatography of polystyrenes

Journal of Chromatography. a
M PetroJean M J Fréchet

Abstract

Gradient elution separations of polystyrene standards in a monolithic molded 50 x 8 mm I.D. poly(styrene-co-divinylbenzene) rod column and in 50 x 8 mm I.D. and 30 x 4.1 mm I.D. columns packed with porous and non-porous poly(styrene-co-divinylbenzene) beads has been carried out. All of these separation media differ in shape and porosity. Excellent separations of eight polystyrene standards were achieved with both the molded monolithic rod and porous beads at moderate flow-rates. However, the monolithic medium proved to be superior for high-speed separations using very steep gradients at a flow-rate of 20 ml/min. Three polystyrene standards were separated in the rod column within 4 s. The separation in the column packed with non-porous beads was poor at flow-rates of 2-8 ml/min, while higher flow-rates led to an unacceptably high back pressure.

Citations

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·D Josić, A Buchacher
May 1, 2012·Journal of Chromatography. a·Yongqin LvFrantisek Svec
Apr 6, 2012·Journal of Pharmaceutical and Biomedical Analysis·Szabolcs FeketeDavy Guillarme
Jul 16, 2008·Journal of Separation Science·Jirí Urban, Pavel Jandera
Sep 10, 2008·Electrophoresis·Amparo Cantó-MirapeixErnesto F Simó-Alfonso
May 17, 2012·Journal of Separation Science·Alexander KorolevAlexander Kurganov
Oct 10, 2013·Journal of Separation Science·Elena MaksimovaTatiana Tennikova
Aug 22, 2006·Journal of Chromatography. a·Monique van Hulst, Peter Schoenmakers
May 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Karel StulíkPavel Coufal
Sep 10, 2004·Journal of Separation Science·Frantisek Svec
Jun 15, 2006·Rapid Communications in Mass Spectrometry : RCM·Mariola BatyckaBenedikt M Kessler
Apr 22, 2015·Journal of Separation Science·Alexander KurganovAnastasiia Kanateva
Dec 17, 2014·Journal of Chromatography. a·Axel VaastSebastiaan Eeltink
Jun 6, 2017·Electrophoresis·Frantisek Svec
Jan 29, 2002·Analytical Chemistry·Fuquan Yang, Yoichiro Ito

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.