Molecular adaptations to phosphorus deprivation and comparison with nitrogen deprivation responses in the diatom Phaeodactylum tricornutum

PloS One
Leila AlipanahAtle M Bones

Abstract

Phosphorus, an essential element for all living organisms, is a limiting nutrient in many regions of the ocean due to its fast recycling. Changes in phosphate (Pi) availability in aquatic systems affect diatom growth and productivity. We investigated the early adaptive mechanisms in the marine diatom Phaeodactylum tricornutum to P deprivation using a combination of transcriptomics, metabolomics, physiological and biochemical experiments. Our analysis revealed strong induction of gene expression for proteins involved in phosphate acquisition and scavenging, and down-regulation of processes such as photosynthesis, nitrogen assimilation and nucleic acid and ribosome biosynthesis. P deprivation resulted in alterations of carbon allocation through the induction of the pentose phosphate pathway and cytosolic gluconeogenesis, along with repression of the Calvin cycle. Reorganization of cellular lipids was indicated by coordinated induced expression of phospholipases, sulfolipid biosynthesis enzymes and a putative betaine lipid biosynthesis enzyme. A comparative analysis of nitrogen- and phosphorus-deprived P. tricornutum revealed both common and distinct regulation patterns in response to phosphate and nitrate stress. Regulation of ce...Continue Reading

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Citations

Jun 26, 2020·Frontiers in Plant Science·Gianluca Dell'AquilaUwe G Maier
Nov 30, 2019·Scientific Reports·Luisana AvilanBrigitte Gontero
May 23, 2019·Applied Microbiology and Biotechnology·Yongting QiuRuibo Ji
Dec 8, 2020·Preparative Biochemistry & Biotechnology·Rıza Akgülİnci Tüney Kızılkaya
Jan 26, 2020·Trends in Biotechnology·Thomas ButlerSeetharaman Vaidyanathan
May 11, 2021·Frontiers in Plant Science·Nolwenn GuéguenEric Maréchal

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Datasets Mentioned

BETA
GSE66063

Methods Mentioned

BETA
Feature Extraction
RNAseq
PCR
Fluorescence
confocal microscopy
RNA-Seq
deacylation
metabolome profiling

Software Mentioned

Affinity Designer
LinRegPCR
GeneDoc
MetAlign
Limma ( Linear Models for Microarray Data )
Agilent ChemStation
MultiExperiment Viewer
Agilent Feature Extraction
OpenChrom Community Edition Synge
Limma Package

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