Molecular analyses of Vibrio cholerae O1 clinical strains, including new nontoxigenic variants isolated in Mexico during the Cholera epidemic years between 1991 and 2000.

Journal of Clinical Microbiology
Marcial Leonardo Lizárraga-Partida, Marie-Laure Quilici

Abstract

We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding cholera toxin. As previously reported, we characterized two populations among toxigenic strains, which were present from the first year of the epidemic. BglI rRNA analysis revealed that these strains had ribotype profiles, denoted M5 and M6 in our study, that were identical to those previously designated Koblavi B5 or Popovic 5 and Popovic 6a or Tamayo B21a, respectively. Ribotype M5 was isolated between 1991 and 1993. This ribotype had a low level of genetic variation as detected by pulsed-field gel electrophoresis (PFGE). Ribotype M6 persisted from 1991 to 2000. However, PFGE profiles suggested that two epidemiologically unrelated strains coexisted within this single ribotype from 1995 until the end of the epidemic. We identified three new BglI ribotypes, Mx1, Mx2, and Mx3, from nontoxigenic V. cholerae O1 strains isolated between 1998 and 2000; one of them grouped strains positive for the toxin-coregulated pilus isla...Continue Reading

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Citations

Apr 21, 2012·Journal of Clinical Microbiology·Munirul AlamAlejandro Cravioto
Dec 18, 2010·Future Microbiology·Tushar Suvra BhowmickB L Sarkar
Feb 24, 2016·Frontiers in Microbiology·Goutam ChowdhuryThandavarayan Ramamurthy
Feb 5, 2015·International Journal of Environmental Health Research·Abraham GuerreroMarcial Leonardo Lizárraga-Partida
Nov 15, 2019·Journal of Food Protection·Claudia VillicañaJosefina León-Félix
Nov 11, 2017·Science·Daryl DommanNicholas R Thomson
Aug 10, 2019·Frontiers in Public Health·Thandavarayan RamamurthyGopinath Balakrish Nair

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