Molecular analysis of an operon in Bacillus subtilis encoding a novel ABC transporter with a role in exoprotein production, sporulation and competence.

Microbiology
S LeskeläM Sarvas

Abstract

The levels of exoamylase and other exoenzymes of Bacillus subtilis are pleiotropically decreased by the ecs-26 (prs-26) and ecs-13 (prs-13) mutations. These mutations also cause a competence- and sporulation-deficient phenotype. In the present work, the ecs locus, which has been defined by the ecs-26 and ecs-13 mutations, was cloned and sequenced. Sequence analysis revealed a putative operon of three ORFs (ecsA, ecsB and ecsC). ecsA can encode a putative polypeptide of 248 amino acid residues containing an ATP-binding site. The polypeptide shows about 30% sequence similarity with the ATP-binding components of numerous membrane transporters of the ABC-type (ATP-binding cassette transporters or traffic ATPases). The ecs-26 mutation was found to result from a transition of one base pair chaning the glycine164 of EcsA to a glutamic acid residue in the vicinity of the putative ATP-binding pocket. ecsB was predicted to encode a hydrophobic protein with six membrane-spanning helices in a pattern found in other hydrophobic components of ABC transporters. The properties deduced for the ecsA and ecsB gene products are consistent with the interpretation that ecs encodes a novel ABC-type membrane transporter of B. subtilis. The third ORF, ...Continue Reading

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Citations

Nov 8, 2006·Applied and Environmental Microbiology·Elise DarmonJan Maarten van Dijl
Jun 4, 2004·Journal of Bacteriology·Steven S BrandaRoberto Kolter
Mar 29, 2008·BMC Bioinformatics·Miaomiao ZhouRoland J Siezen
Jul 13, 2011·PloS One·Aleksandra M MirończukAkos T Kovács
Nov 17, 2012·PloS One·Jessica C ZweersEmma L Denham
Jun 11, 2004·Letters in Applied Microbiology·H WestersJ M van Dijl
Jun 26, 2003·Molecular Microbiology·Haike AntelmannJan Maarten van Dijl
May 1, 2008·Microbial Biotechnology·Aleksandra M MirończukOscar P Kuipers
Oct 28, 2015·PloS One·Wout Overkamp, Oscar P Kuipers
Dec 20, 2016·PloS One·Jennifer C Chang, Michael J Federle
Mar 27, 1999·Journal of Molecular Biology·Y QuentinF Denizot

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