Sep 18, 2004

Molecular analysis of APC promoter methylation and protein expression in colorectal cancer metastasis

Carcinogenesis
Jie ChenMatthias P A Ebert

Abstract

APC (adenomatous polyposis coli) promoter methylation has been linked to the early development of colorectal cancers. However, the role of APC methylation and its effect on protein expression in colon cancer metastasis is largely unknown. In this study, we investigated APC promoter methylation by Methylight analysis and analysed the APC protein levels by immunohistochemistry and western blot analysis in 24 liver metastasis and 39 primary colorectal cancers. Promoter methylation of the APC gene was found to be a frequent event in liver metastasis (10/24) and significantly more frequent compared with primary colorectal cancer (7/39, P = 0.047). APC methylation was not found in 14 matched normal colon tissues. APC protein was detected in the cytoplasm of primary and metastatic cancer cells and non-tumorous colon epithelium. By western blot analysis, APC protein levels were found to be decreased in primary tumour tissues compared with the normal colon mucosa. In contrast, APC protein levels were not decreased in the cancer cells that had metastasized to the liver. APC protein levels were independent of the presence of APC promoter methylation or gene mutations. In summary, APC promoter methylation is a frequent epigenetic alteratio...Continue Reading

Mentioned in this Paper

Gene Expression Regulation, Neoplastic
Immunohistochemistry
Protein Methylation
APC gene
Neoplasms
DNA Methylation
Tumor Lysis Syndrome
Malignant Tumor of Colon
Promoter
Gene Mutation

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