Molecular Analysis of Bacterial Communities in a Three-Compartment Granular Activated Sludge System Indicates Community-Level Control by Incompatible Nitrification Processes

Applied and Environmental Microbiology
W E HolbenY Suwa

Abstract

Bacterial community structure and the predominant nitrifying activities and populations in each compartment of a three-compartment activated sludge system were determined. Each compartment was originally inoculated with the same activated sludge community entrapped in polyethylene glycol gel granules, and ammonium nitrogen was supplied to the system in an inorganic salts solution at a rate of 5.0 g of N liter of granular activated sludge-1 day-1. After 150 days of operation, the system was found to comprise a series of sequential nitrifying reactions (K. Noto, T. Ogasawara, Y. Suwa, and T. Sumino, Water Res. 32:769-773, 1998), presumably mediated by different bacterial populations. Activity data showed that all NH4-N was completely oxidized in compartments one and two (approximately half in each), but no significant nitrite oxidation was observed in these compartments. In contrast, all available nitrite was oxidized to nitrate in compartment three. To study the microbial populations and communities in this system, total bacterial DNA isolated from each compartment was analyzed for community structure based on the G+C contents of the component populations. Compartment one showed dominant populations having 50 and 67% G+C content...Continue Reading

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Citations

Sep 7, 2001·Annual Review of Microbiology·G A Kowalchuk, J R Stephen
Feb 4, 2009·Biotechnology and Bioengineering·Julio PérezJan-Ulrich Kreft
Oct 9, 2003·Reviews on Environmental Health·Itziar AlkortaCarlos Garbisu
Oct 20, 2005·Journal of Bioscience and Bioengineering·K Watanabe, P W Baker
Apr 20, 2006·Trends in Microbiology·Engràcia CostaJan-Ulrich Kreft

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