Molecular analysis of CaMnt1p, a mannosyl transferase important for adhesion and virulence of Candida albicans

Proceedings of the National Academy of Sciences of the United States of America
Ed T BuurmanN A Gow

Abstract

There is an immediate need for identification of new antifungal targets in opportunistic pathogenic fungi like Candida albicans. In the past, efforts have focused on synthesis of chitin and glucan, which confer mechanical strength and rigidity upon the cell wall. This paper describes the molecular analysis of CaMNT1, a gene involved in synthesis of mannoproteins, the third major class of macromolecule found in the cell wall. CaMNT1 encodes an alpha-1, 2-mannosyl transferase, which adds the second mannose residue in a tri-mannose oligosaccharide structure which represents O-linked mannan in C. albicans. The deduced amino acid sequence suggests that CaMnt1p is a type II membrane protein residing in a medial Golgi compartment. The absence of CaMnt1p reduced the ability of C. albicans cells to adhere to each other, to human buccal epithelial cells, and to rat vaginal epithelial cells. Both heterozygous and homozygous Camnt1 null mutants of C. albicans showed strong attenuation of virulence in guinea pig and mouse models of systemic candidosis, which, in guinea pigs, could be attributed to a decreased ability to reach and/or adhere internal organs. Therefore, correct CaMnt1p-mediated O-linked mannosylation of proteins is critical fo...Continue Reading

References

Jun 1, 1992·Antimicrobial Agents and Chemotherapy·R F Hector, K Schaller
Dec 1, 1992·Current Opinion in Cell Biology·G W Hart
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A HäuslerP W Robbins
Jan 1, 1991·Annual Review of Microbiology·J E Cutler
Jan 1, 1991·Methods in Enzymology·P PhilippsenC Scherf
Aug 1, 1990·Trends in Biochemical Sciences·N Jentoft
Jan 1, 1986·Molecular and Cellular Biology·M B KurtzD R Kirsch
Jan 1, 1987·Molecular and Cellular Biology·R KellyD R Kirsch
Apr 1, 1970·Journal of Bacteriology·W L McLellanJ O Lampen
Sep 1, 1982·Journal of General Microbiology·N A Gow, G W Gooday
Jan 1, 1982·Annual Review of Biochemistry·E CabibB Bowers
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·C E BulawaJ M Becker
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·C E BallouG Ball
May 1, 1993·Trends in Microbiology·R A Calderone
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·C L YipV L MacKay
Jan 1, 1993·Canadian Journal of Microbiology·C W White, E S Jacobson
Apr 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Herscovics, P Orlean
Feb 1, 1996·Glycoconjugate Journal·E F HounsellD V Renouf
Mar 1, 1997·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·Y Fukazawa, K Kagaya
Feb 1, 1996·International Journal of Antimicrobial Agents·F C Odds

❮ Previous
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Citations

Mar 16, 2001·FEMS Microbiology Reviews·F Navarro-GarcíaJ Pla
Jan 8, 1999·Biochimica Et Biophysica Acta·M LussierH Bussey
Dec 4, 2001·FEMS Microbiology Letters·J M BainN A Gow
Jul 31, 1999·FEMS Microbiology Letters·R Diez-OrejasM Sánchez-Pérez
Jul 4, 2001·Trends in Microbiology·R A Calderone, W A Fonzi
Feb 28, 2006·Revista iberoamericana de micología·Rosângela V AndradeMaria Sueli S Felipe
Aug 23, 2002·Microbes and Infection·Alessandra A CostaCélia M de Almeida Soares
Jul 26, 2002·Molecular Microbiology·Frédérique MoyrandGuilhem Janbon
Apr 9, 2010·Clinical Microbiology Reviews·Jacqueline M Achkar, Bettina C Fries
Aug 24, 2004·Infection and Immunity·Jason M RauceoPeter N Lipke
Jan 29, 2011·BMC Genomics·Ana Karina R AbadioMaria Sueli S Felipe
May 13, 2010·Biological & Pharmaceutical Bulletin·Nobuyuki Shibata, Yoshio Okawa
Jun 26, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Nobuyuki ShibataShigeo Suzuki
Nov 10, 2009·Future Microbiology·Héctor M Mora-MontesEverardo López-Romero
Nov 12, 2010·Medical Mycology·Sanjay H ChotirmallNoel G McElvaney
Jan 17, 2016·Pathogens and Disease·Lindsay C DuttonAngela H Nobbs
Mar 8, 2003·Molecular Microbiology·Jonathan D CroweNuala A Booth
Jul 20, 2010·Yeast·Alice G SorgoFrans M Klis
Oct 16, 2013·Molecular Microbiology·Rebecca A Hall, Neil A R Gow
Nov 24, 2004·Microbes and Infection·Marcia R PintoEliana Barreto-Bergter
Nov 11, 2006·Microbes and Infection·Rafael C Prados-RosalesAntonio Di Pietro
Feb 14, 2012·Biochemical and Biophysical Research Communications·Diana F Díaz-JiménezArturo Flores-Carreón

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